【摘要】 目的建立颈舒片中士的宁和马钱子碱的含量测定方法。方法以乙腈-0.01mol·L·-1庚烷磺酸钠与0.02mol·L-1磷酸二氢钾等量混合液(用磷酸调pH至2.6)(19∶81)为流动相,柱温:30℃;检测波长260nm,流速0.9mL·min-1。结果士的宁在11.2~179.2μg·mL-1、马钱子碱在10.6~169.6μg·mL-1浓度范围内线性良好,相关系数分别为r=0.9999、r=0.9990,回收率分别为98.2%、RSD=0.74%,99.8%、RSD=1.4%。结论本法专属性强,操作简便,可作为腰腿痛片的质量控制方法。更多还原

【Abstract】 OBJECTIVE To establish a method for the determination of Strychine and Brucine in Jingshu Tablets by HPLC.METHODS The samples were separated on a Hypersil Gold C18(4.6mm ×150mm,5μm) column at the flow rate of 0.9mL·min- 1and the column temperature was 30℃.Acetonitrile and a mixed solution of equal amount of 0.01mol·L- 1Sodium heptanesulfonate and 0.02mol·L- 1potassium dihydrogen phosphate(pH was adjusted to 2.6 by phosphoric acid)(1 ∶ 81) was used as the mobile phase,the detection wavelength was 240nm.RESULTS The method had good linear relationship within the range of 11.2 ~ 179.2μg · mL- 1(r = 0.9999) for Strychnine,and 10.6 ~ 169.6μg·mL- 1(r = 0.9998) for Brucine.The average recoveries and RSD were 98.2% and 0.74%(n = 9) for Strychnine,99.8% and 1.4%(n = 9) for Brucine.CONCLUSION The method is specific,well-done and can be used for the quality control of Jingshu Tablets.更多还原