【摘要】 以荔枝转基因原生质体再生的胚性愈伤组织(TPEC)细胞系和龙眼‘红核子’品种的松散型胚性愈伤组织(EC)为材料,采用PA-4000电融合仪进行原生质体电融合参数的研究.结果表明:荔枝TPEC与龙眼EC原生质体融合的适宜电融合参数为原生质体密度5×105个·mL-1,交流电场频率1.0 MHz、交变电场场强50 V·cm-1,作用时间60 s,直流脉冲强度600 V·cm-1、脉冲持续时间10 ms,脉冲2-3次.在此条件下,融合率可达30%以上.更多还原

【Abstract】 Litchi embryogenic callus derived from transgenic protoplasts(TPEC) and longan embryogenic callus(EC) were used as the material,the protoplast electrofusion parameters were studied by means of Cyto pulse PA-4000 cell electrofusion apparatus.The results showed that the suitable fusion parameters between the protoplasts from TPEC in litchi and the friable embryogenic calli in longan were as follows:the protoplast density of 5 × 105protoplasts·mL-1,1.0 MHz AC frequency and 50 V·cm-1AC voltage for60 s,600 V·cm-1DC strength for 10 ms,the pulse for 2-3 times.The rate of the protoplast fusion reached more than 30%.更多还原