【摘要】 目的 描述龟分枝杆菌蛋白质分泌活动,为其致病性及检测方法研究提供依据。方法 采用弱阳离子交换蛋白质芯片(WCX2)捕获并应用表面增强激光解析-电离飞行时间质谱(SELDI-TOF-MS)技术,检测11株龟分枝杆菌临床分离株及ATCC19977参考株培养7d的Middlebrook 7H9培养滤液蛋白和Middlebrook 7H9培养液蛋白,描述性分析龟分枝杆菌培养滤液蛋白质组。结果 相对Middlebrook 7H9培养液,在11株龟分枝杆菌7d的Middlebrook 7H9培养滤液检测到49～101种差异蛋白,相对分子质量为1101～3953,丰度水平为84～7238相对强度(用质谱峰面积计算)。其中32种差异蛋白在11株中均有表达,相对分子质量为1108～3953,丰度水平为98～7231相对强度。结论 龟分枝杆菌在体外生长过程中可分泌多种小分子蛋白质,其特性和作用有待进一步研究。更多还原

【Abstract】 Objective To describe the protein secretion activity of M.chelonae,and to provide the research basis for its pathogenicity and detection methods. Methods With weak cation exchange protein chip(WCX2),Middlebrood 7 H9 culture filtrate proteins for 7 days’ culture and Middlebrook 7 H9 culture proteins of 11 M.chelonae clinical isolates and ATCC19977 reference strain,were detected by surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS),the M.chelonae,culture filtrate proteins were analyzed descriptively. Results 49-101 kinds of differential proteins were detected in 11 M.chelonae Middlebrook 7 H9 culture filtrate for 7 days’ culture,the relative molecular mass ranged from 1101-3953,and the abundance level in relative strength were 84-7238.Thirty-two kinds of differential proteins were detected in 11 M.chelonae,the molecular mass ranged from 1108-3953,and the abundance level in relative strength were 98-7231. Conclusion A variety of small proteins were secreted by M.chelonae in the process of growth in vitro,while there characteristics and effects needs further research.更多还原