【摘要】 目的探索储存红细胞添加NO供体SNP后血液流变学各项指标以及红细胞内胞浆NO、总NO、Hb结合NO含量变化,并与新鲜红细胞内3种NO含量比较,探索其可能的临床意义。方法血样采自10名健康献血者,制成悬浮红细胞后分为新鲜红细胞组和储存红细胞组。新鲜红细胞组滤除白细胞后检测红细胞内3种NO含量。储存红细胞组储存d20时滤除白细胞,分为2部分,1部分与终浓度分别为1、10μmol/L的SNP孵育,检测流变学各项指标;另1部分与终浓度分别为0.2、1、5、25μmol/L的SNP孵育,检测红细胞内3种NO含量。结果当储存红细胞添加1μmol/L SNP时,各项流变学指标与未加SNP组相比均无统计学意义(P>0.05);当添加10μmol/L SNP时,红细胞低切粘度、中切粘度、还原低切粘度、还原中切粘度、聚集指数、电泳指数分别为4.16±0.74、2.94±0.41、9.41±1.19、5.92±0.48、1.64±0.10、4.30±0.28,均显著低于未加SNP组(P<0.05)。新鲜红细胞内3种NO含量分别是11.09±2.95、16.63±4.46、5.54±3.53,储存红细胞(未加SNP组)3种NO含量分别是4.93±1.01、7.20±0.93、2.27±1.18,后者3种NO含量均明显低于前者(P<0.05)。当储存红细胞与0.2μmol/L SNP孵育时,3种NO含量与未加SNP组比较均无统计学差异(P>0.05);当增加至1μmol/L时,3种NO含量分别是5.93±1.41、9.57±1.56、3.74±1.36,均显著高于未加SNP组(P<0.05);当增加至5μmol/L时,总NO、Hb结合NO含量分别为8.45±2.12、3.29±1.54,继续增加至25μmol/L时,总NO、Hb结合NO含量分别为8.86±1.16、3.65±0.91,均明显高于未加SNP组(P<0.05)。结论添加适当浓度NO供体SNP可以明显改善储存红细胞变形性,显著增加红细胞内NO含量。更多还原

【Abstract】 Objective To detect hemorheology and cytoplasm NO,total NO and NO-hemoglobin levels in erythrocytes after adding different concentrations of SNP to stored suspension red blood cells( RBCs). Methods Blood samples were collected from 10 healthy donors,then divided into fresh and 20 days stored suspension RBCs groups. Hemorheology was assayed by cone-plate rotating structure principle. Cytoplasm NO and total NO were detected by colorimetric analysis. The level of NO-hemoglobin was equal to the level of total NO minus cytoplasm NO. The NO levels of fresh red blood cells were detected with above methods. At 20 days,stored red blood cells were divided into two parts. Part one was incubated with SNP at 1 μmol / L and 10 μmol / L concentrations for 1 hour and hemorheology was assayed with the above method. Part two was incubated with SNP at 0. 2,1,5 and 25 μmol / L concentrations for 1 hour and the NO levels were detected with above methods. Results When stored erythrocytes were incubated with 10 μmol / L SNP,the viscosity in low,middle shear,index of suspension erythrocytes aggregation and electrophoresis index were 4. 16 ± 0. 74,2. 94 ± 0. 41,9. 41 ± 1. 19,5. 92 ± 0. 48,1. 64 ± 0. 10,and 4. 30 ± 0. 28 respectively. Values obtained were significantly lower than the group without SNP addition( P < 0. 05),while 1 μmol / L SNP showed no effect on hemorheology( P > 0. 05). For fresh red blood cells,the levels of cytoplasm NO,total NO and NO-hemoglobin were 11. 09 ± 2. 95,16. 63 ± 4. 46,5. 54 ± 3. 53,and stored cells were 4. 93 ± 1. 01,7. 20 ± 0. 93,2. 27 ± 1. 18,respectively. The stored groups had NO levels significantly lower than fresh ones. At 0. 2 μmol / L SNP incubation,the three NO levels did not change compared with the 20 days stored group( P > 0. 05) and when SNP concentration went up to 1 μmol / L,the three NO levels were 5. 93 ± 1. 41,9. 57 ± 1. 56,3. 74 ± 1. 36. Significant differences were found compared to the control group( P < 0. 05),when SNP was added to 5 μmol / L,the total NO and NOhemoglobin levels were 8. 45 ± 2. 21,3. 29 ± 1. 54 and significantly higher than the control group( P < 0. 05). When adding SNP to 25 μmol / L,the total NO and NO-hemoglobin levels were 8. 86 ± 1. 16,3. 65 ± 0. 91,and significantly higher than the control group( P < 0. 05). The cytoplasm NO did not increase with the rising of SNP concentrations. Conclusion Addition of an appropriate concentration of SNP could significantly improvethe deformability and the NO levels of stored red blood cells.更多还原