【摘要】 目的探讨口腔癌患者唾液中过氧化氢酶(CAT)的含量及其对口腔癌的辅助诊断价值,以及与淋巴结转移和病理分级的关系。方法选择首次住院治疗的口腔癌患者97例,正常对照组50例。口腔癌患者组于治疗前取混合唾液4ml,正常对照组取混合唾液4ml。样本采集后立即在4℃条件下离心(3000r/min)20 min,提取上清液2ml,采用可见光分光光度法测定所有唾液样本中CAT的含量。结果口腔癌患者治疗前唾液中CAT含量明显高于正常对照组,独立样本T检验显示两者间差异具有统计学意义(P<0.01)。口腔癌患者唾液中CAT的含量与肿瘤淋巴结转移、病理分级和发病部位无关(P值分别为0.772、0.636、0.277)。结论口腔癌患者治疗前唾液中CAT含量明显高于正常对照组。唾液中CAT含量的检测对口腔癌患者有一定的辅助诊断价值。更多还原

【Abstract】 Objective To determine the levels of catalase(CAT) in saliva of patients with oral cancer,and to investigate its diagnostic value and the relationship with lymph node metastasis and pathological grade. Methods Ninetyseven patients with oral cancer hospitalized in Beijing Stomatological Hospital and fifty healthy individuals were included. 4 ml mixed unstimulated saliva was collected from these patients and the healthy controls before therapy. The CAT levels of all saliva samples were detected by visible spectrophotometric method. The data were statistically analyzed. Results The saliva CAT level was significantly higher in patients with oral cancer than that in the control( P < 0. 01). There was no significant difference between saliva CAT level and lymph node metastasis,pathological grade,clinical stage and the location of tumor. Conclusion The saliva CAT may prove to be useful tumor marker in oral cancer and serve as an adjunct in diagnosis.更多还原