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壳聚糖诱导芦笋对茎枯病的抗性研究

Study on Resistance of Asparagus officinalis L. Against Stem Blight Induced by Chitosan

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【作者】 秦力菅文磊崔日秀陈景丽卢钢

【Author】 QIN Li;JIAN Wen-lei;CHOE Il-su;CHEN Jing-li;LU Gang;College of Agriculture and Biotechnology,Zhejiang University;College of Life Science,Kim Il-sung University;

【机构】 浙江大学农业与生物技术学院朝鲜金日成综合大学生命科学院

【摘要】 以芦笋为试材,对接种茎枯病病原菌后的芦笋在不同时期喷施壳聚糖处理,并研究了发病情况、叶片光合作用与抗氧化酶活性的变化。结果表明:喷施壳聚糖后,芦笋茎枯病发病率及发病指数均显著降低,且以接种病原菌后立即喷施壳聚糖效果最好,芦笋体内过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、愈创木酚过氧化物酶(G-POD)、多酚氧化酶(PPO)的活性升高,接种病原菌造成的拟叶光合速率和气孔导度下降现象也得到明显缓解。表明壳聚糖能诱导芦笋茎枯病抗性,降低病原菌对其造成的伤害。

【Abstract】 Taking Asparagus officinalis L.as material,the different asparagus cultivars were used and their seedlings were inoculated with Phomopsis aspasagi(Sacc)Bubak.The chitosan were sprayed in different periods after inoculation.Morbidity and disease index were surveyed after the treatment.The changes of antioxidant enzyme activities were compared.The photosynthetic rate and stomatal conductance of intended leaves were also determined.The results showed that the disease index significantly reduced with chitosan-supply,and the best effect obtained byspraying chitosan immidietly after inoculation.The activity of catalase(CAT),guaiacol peroxidase(G-POD),superoxide dismutase(SOD),polyphenoloxidase(PPO)activity of Asparagus officinalis L.increased.On the other hand,the decline of photosynthetic rate and stomatal conductance caused by inoculation was suppressed after supplied with the chitosan.It could be concluded that chitosan could induce systemic resistance on asparagus against stem blight,reduce the damage caused by pathogens,and resulted in beneficial effects on asparagus production.

【关键词】 芦笋壳聚糖茎枯病抗性
【Key words】 Asparagus officinalis L.chitosanstem blightresistance
【基金】 国家“十二五”科技支撑计划资助项目(2011BAD12B04);国家公益性行业(农业)科研专项资助项目(201003074-3);浙江省重点资助项目(2012C12012-3)
  • 【文献出处】 北方园艺 ,Northern Horticulture , 编辑部邮箱 ,2014年20期
  • 【分类号】S436.44
  • 【被引频次】3
  • 【下载频次】117
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