【摘要】 [目的]了解草鱼♀×鳡♂杂交F1代的生化遗传特性。[方法]应用聚丙烯酸胺凝胶垂直板电泳技术,对草鳡杂交F1代的9种组织(心、脑、眼、肝、肾、脾、鳍、肌肉、血浆)的3种同工酶(LDH、EST、MDH)进行了分析,同时比较了其同工酶及蛋白质与亲本草鱼、鳡的差异。[结果]草鳡杂交F1代的3种同工酶具有不同程度的组织特异性,其同工酶和蛋白质与亲本之间存在明显差异。[结论]这些差异可作为鉴别草鳡杂交鱼及亲本的标志。更多还原

【Abstract】 [Objective] In order to understand the genetic characteristics of hybrid F1( C. idellus ♀ × bambusa ♂). [Method]By means of vertical polyacrylamide gel electrophoresis,three isozymes( LDH,EST and MDH) from nine tissues( heart,brain,eyes,liver,kidney,spleen,fin,muscle,plasma) of the F1 hybrid were studied,at the same time,the isozyme and protein of the F1 hybrids were compared with grass carp and E. bambusa. [Result]The results showed that three isozymes presented obvious tissue specificity; The isozyme and protein of F1 hybrid exists obvious difference between with its parents. [Conclusion] These differences can be used as identification of F1 hybrid and its parents.更多还原