【摘要】 [目的]探索滑菇Pholiota nameko SW-01菌株产漆酶的最佳营养条件及其酶学性质。[方法]首先选取不同的碳源、氮源、pH、金属离子进行单因素试验,得到最佳培养基成分,进行四因素三水平的响应面分析。在酶学性质试验中,设置不同温度及pH,测定漆酶的最适反应温度、pH及热稳定性、pH稳定性。[结果]最适的碳源和氮源分别为葡萄糖和蛋白胨,最适pH为5,Cu2+能显著提高漆酶的产量。响应面分析结果表明,最佳的产漆酶条件为葡萄糖42.50 g/L,蛋白胨2.10 g/L,pH 5.47,Cu2+浓度1.67 mmol/L,在此条件下漆酶活性为943.27 U。在酶学性质试验中,漆酶的最适反应温度为30℃,最适反应pH为4.8。[结论]条件优化能显著提高滑菇漆酶产量。稳定性试验表明,漆酶对温度较敏感,对pH反应不敏感,在弱酸环境中能发挥较高的活性。更多还原

【Abstract】 [Objective] The optimum nutritional conditions for laccase production and properties of Pholiota nameko SW-01 were studied.[Method]The optimum conditions(carbon resources,nitrogen resources,pH and metal ions) for the laccase production and the interaction of the four variables(glucose,peptone,pH and copper ion) were processed using one-single-factor experiment and response surface methodology,respectively.Different temperatures and pH levels were proceed to evaluate the properties of laccase.[Result] The optimum carbon resource was glucose,the optimun nitrogen resource was peptone,optimum pH acid was 5 and the suitable metal ion was copper ion.Optimum conditions for laccase production were as follows:glucose 42.50 g / L,peptone 2.10 g / L,pH 5.47 and copper ion 1.67 mmol / L,and the maximum laccase activity reached 943.27 U.The laccase showed an optimal reaction temperature of 30 ℃ and pH of 4.8.[Conclusion]Under the optimum conditions,the laccase production was significantly increased.And the stability experiments showed that laccase was sensitive with temperature and non-sensitive with pH,and had a good laccase activity in acid conditions.更多还原