【摘要】 目的建立快速检测葡萄球菌肠毒素B(staphylococcal enterotoxin B,SEB)基因的聚合酶链反应(PCR)方法。方法①根据SEB基因的序列,设计PCR引物特异性扩增靶基因片段。通过对1株产SEB金黄色葡萄球菌和9株对照菌株进行PCR检测,评价该方法的特异性;通过对产SEB金黄色葡萄球菌菌株做10倍系列稀释后进行PCR检测,评价该方法的敏感性;②分析30株金黄色葡萄球菌SEB基因的携带情况。结果①建立PCR方法快速检测SEB基因,扩增产物长度为494 bp。PCR反应体系中有26 CFU的产SEB金黄色葡萄球菌即可检出SEB基因;②30株分离的金黄色葡萄球菌中有2株携带有SEB基因。结论 PCR法可以快速、敏感地检测SEB基因,为金黄色葡萄球菌食物中毒诊断提供依据。更多还原

【Abstract】 [Objective]To establish a PCR assay for the rapid and sensitive detection of Staphylococcal enterotoxin B(SEB) gene.[Methods]①According to the sequences of the SEB gene,primers were selected and were used to amplify SEB gene.To evaluate the specificity of the assay,1 strain of SEB positive Staphylococcus aureus and 9 strains of non-Staphylococcus aureus were tested by PCR.To evaluate the sensitivity of the assay,the strain of SEB positive Staphylococcus aureus was 10-fold serially diluted and was amplified by PCR.②The SEB gene in 30 strains of Staphylococcus aureus were detected by PCR.[Results] ①The PCR assay for the rapid and sensitive detection of SEB gene was well established.The length of amplification was 494 bp.The detection limit for SEB gene was 26 CFU;② Among 30 strains of Staphylococcus aureus,2 strains were positive for SEB gene.[Conclusion]The PCR assay can rapidly and exactly detect the SEB gene,and can provide evidence for the rapid diagnosis of Staphylococcal food poisoning.更多还原