【摘要】 目的:采用星点设计-效应面法对当归的提取工艺进行优化。方法:在单因素考察的基础上,以甲醇浓度、溶剂用量、超声提取时间为自变量,阿魏酸、藁本内酯得率的"归一化"值为因变量,通过对自变量各水平进行二项式拟合,运用效应面法优化最佳工艺。结果:最优工艺条件为:加入30倍量70%甲醇,超声提取1次,时间30min;总评"归一值"的预测值与理论值偏差1.23%。结论:采用星点设计-效应面法优化当归提取的工艺条件,方法简便可靠,预测性较优。更多还原

【Abstract】 Objective: To optimize the extracting technology from Angelica sinensis by central composite design-response surface methodology. Methods: On the basis of the single factor,independent variables were ethanol concentrations,solvent ratio and ultrasonic time,while dependent variable was the OD value of extraction rates of ferulic acid and liqustilide. A two-order polynomial equation was fitted to estimate the relationship between independent and dependent variables. Response surface method was used to optimize the extracting process. Results: The optimum extraction conditions for Angelica sinesis were obtained as follows: the extracting solvent was methanol concentrations of 70%,30 fold solvent,extracting for once and for 40 minutes. The deviation between observed and predicted values was 1. 23%. Conclusion: The result indicates that the central composite design and response surface methodology is simple,convenient and reliable for optimizing the extraction process of Angelica sinesis with higher precision.更多还原