【摘要】 目的:阐明结核分支杆菌耐异烟肼临床分离株katG、inhA和ahpC基因突变特点。方法:对75株耐异烟肼临床分离株和45株异烟肼敏感临床分离株katG、inhA和ahpC基因PCR并测序。结果:45株异烟肼敏感菌株中除18株katG基因存在R463L突变外无其它突变。75株耐异烟肼菌株中未发现katG、inhA和ahpC基因完全缺失。分别有68株、17株和8株分别发生katG、inhA和ahpC基因突变,43株(57.3%)发生katG基因S315位突变,结合菌株耐药程度,异烟肼高度耐药株(INH≥10μg/ml)S315突变发生率显著高于低度耐药株(INH≥1μg/ml)。33株(44%)katG基因存在R463L突变。结论:katG基因存在R463L突变同时存在于异烟肼敏感菌株和耐药菌株,无差异(P>0.05)提示katG基因R463L突变与异烟肼耐药无关,本地异烟肼耐药株katG基因突变特别是S315突变发生率高,S315突变与异烟肼耐药耐药密切相关且与耐药程度相关。更多还原

【Abstract】 Objective:To elucidate the characteristics of katG,inhA and ahpC gene mutations in isoniazid(INH) resistant isolates of Mycobacterium tuberculosis.Methods:A total of 75 INH-resistant isolates and 45 INH-susceptible isolates of Mycobacterium tuberculosis were analyzed by PCR and sequence analysis of their katG,inhA,and ahpC genes.Results:In addition to R463L mutation in katG gene was found in 18 INH-susceptible isolates,there was no other mutation in the 45 INH-susceptible isolates.Among the 75 INH-resistant isolates,complete deletions of katG,inhA and ahpC genes were not found,mutations in katG,inhA and ahpC were identified in 68,17 and 8 isolates,respectively.S315 mutation were found in katG gene of 57.3%(43/75) of the INH-resistant isolates,high level INH-resistant isolates(INH≥10μg/ml) had higher mutation frequency at S315 than low level INH-resistant isolates(INH≥1μg/ml).R463L was present in 33 of 75 INH-resistant isolates.Conclusions:R463L mutation in katG gene was present in INH-susceptible and INH-resistant isolates,there was no statistically significant difference(P > 0.05).It is suggested that the frequency of the S315 mutation in katG gene was high in the local isolates,S315 mutation was associated with high level INH-resistant isolates.更多还原