【摘要】 目的:分析长春市流感监测情况,探索流感流行特点及病原学变化规律,为预防和控制流感大流行提供科学依据。方法:采集门诊流感病人的咽拭子标本用荧光定量PCR,和狗肾细胞(MDCK)及鸡胚法进行流感病毒检测,病毒分离后采用血凝抑制方法(HI)进行流感病毒型别鉴定。结果:从584例流感样病例标本中检出121份核酸阳性并分离到52株流感病毒,分离阳性率为42.98%其中H3N2型13株(占10.74.%),B-victoria系22株(占18.18%),B-yam agata系17株(占14.05%)。而上一年度以甲型为主尤其是以新甲H1型为主。结论:2011年-2012年度长春市流感的流行情况较为平静,H3N2型,B-victoria系,B-yam agata系成为流感流行的优势株,与上一监测年度(新甲H1,H3N2型,B-victoria系)不同。更多还原

【Abstract】 Objective: To analyze the influenza surveillance situation in Changchun city from 2010 to 2012,explore the influenza epidemic characteristics and etiological changes,and provide scientific basis for prevention and control of influenza pandemic.Methods: The throat swab specimens were collected from outpatient influenza patients and the influenza virus was detected with Real-Time PCR,isolated with MDCK cells and chicken embryo method,and finally identified by hemagglutination inhibition test(HI).Results: From October 2011 to March 2012,one hundred and twenty-one positive samples of nucleic acid were found from 584 influenza-like cases specimens,and 52 strains of influenza viruses were isolated(42.98%),including 13 H3N2 strains(10.74%),22 B-victoria strains(18.18%) and 17 B-yamagata strains(14.05%).New influenza A(H1) was the main type in last surveillance period.Conclusion: The influenza was found calm in Changchun from 2011 to 2012,and the predominant strains were H3N2,B-victoria and B-yamagata,different from the predominant strains(new A(H1),H3N2 and B-victoria) of the last surveillance period.更多还原