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番茄细菌性软腐病病原的分离与鉴定

Isolation and identification of the pathogen causing tomato bacterial soft rot

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【作者】 张国庆； 翟婧； 周青； 王笑； 崔舟琦； 谢关林；

【Author】 ZHANG Guo-qing1,ZHAI Jing1,ZHOU Qing1,WANG Xiao2,CUI Zhou-qi1,XIE Guan-lin1(1Institute of Biotechnology,Zhejiang University,State Key Laboratory of Rice Biology,Key Laboratory of Molecular Biology of Crop Pathogens and Insects Ministry of Agriculture,Hangzhou 310029,China;2Agricultural Technology Center for Extension and Service in Jiangbei,Ningbo 315021,China)

【机构】 浙江大学生物技术研究所水稻生物学国家重点实验室,农业部病虫分子生物学重点开放实验室； 宁波市江北区农技推广服务总站；

【摘要】 <正>Pectobacterium carotovorum subsp.carotovorum(syn.Erwinia carotovora subsp.carotovora)是引起作物腐烂的常见病原细菌,主要侵染亚热带及温带地区的作物。如,大白菜、马铃薯、茄子、辣椒等。近年来,山东省以及浙江省部分地区,番茄陆续出现茎秆严重腐烂现象。发病初期,番茄茎秆、枝条上出现椭圆或条状灰褐色腐斑,然后逐渐扩散、腐烂。湿度大的情况下,植株表皮湿腐,伴随异味产生,茎秆很快腐烂直至整株死亡,给菜农造成

【Abstract】 Tomato bacterial soft rot has been become seriously in recent years in some areas of China.However,little is known about the causal agent.It was isolated and identified for effective management of the disease.The symptoms of the disease were described and the pathogen was tested in clony morphology,pathogenicity,transmission electron microscopy,biolog and fat acid analysis.The results indicated that the causal agent was belonged to Pectobacterium.The 16S rDNA was also sequenced and had 99% similarity with Pectobacterium carotovorum subsp.carotovorum.It was formed in a group with P.c.subsp.carotovorum ATCC15713,LMG2404,DSM30168 and Pectobacterium carotovorum subsp.odoriferum LMG17566,which indicated that the pathogen was Pectobacterium carotovorum.

【关键词】 tomato； bacterial soft rot； biolog； FAMEs； 16S rDNA；
【Key words】 tomato； bacterial soft rot； biolog； FAMEs； 16S rDNA；
【基金】 农业部公益性行业(农业)科研专项(201003029;201003066);国家自然科学基金资助项目(30871655)
  • 【文献出处】 植物病理学报 ,Acta Phytopathologica Sinica , 编辑部邮箱 ,2013年04期
  • 【分类号】S436.412.1
  • 【被引频次】1
  • 【下载频次】421
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