【摘要】 目的研究染料木黄酮(Gen)对β淀粉样肽25-35(Aβ25-35)介导的星形胶质细胞氧化损伤中Nrf2/ARE通路相关因子的调节作用。方法以星形胶质细胞(C6细胞系)作为研究对象,用Aβ25-35染毒制备氧化损伤模型。实验共分4组:对照组、Aβ组(Aβ模型组)、Gen干预组(Gen+Aβ组)及Gen组(Gen单独处理组);采用RT-PCR方法和Western Blot方法检测星形胶质细胞核因子E2相关因子2(Nrf2)、血红素加氧酶(HO-1)、γ-谷氨酰半胱氨酸合成酶催化亚单位(GCLC)及锰超氧化物歧化酶(MnSOD)基因和蛋白的表达。结果与Aβ组比较,Gen干预组和Gen组星形胶质细胞Nrf2、HO-1、GCLC及MnSOD基因和蛋白表达水平显著上调(P<0.05)。结论染料木黄酮可能通过调控Nrf2/ARE通路来拮抗Aβ25-35介导的星形胶质细胞氧化损伤作用。更多还原

【Abstract】 Objective The aim of this study was to investigate the effects of genistein(GEN)on the Nrf2/ARE pathway related factors during protection of oxidative damage induced by β-amyloid peptides(Aβ25-35) in astrocytes(C6 cells).Methods Astrocytes was cultured for 48hrs.Aβ25-35(25μmol/L) was used to establish oxidative damage model in astrocytes.Four groups are control group,Aβ group,Gen-treated group(Gen+Aβ group) and Gen group(solo-Gen group).Gen(50 μmol/L)was applied 2 hrs piror to the addition of Aβ25-35.After 24 hrs pretreatment,cells were harvested for assays.The mRNA and protein expression of nuclear factor erythroid 2-related factor 2(Nrf2),heme oxygenase-1(HO-1),catalytic subunit of glutamate-cysteine ligase(GCLC)and manganese superoxide dismutase(MnSOD)in astrocytes were tested by RT-PCR and Western Bolt.Results Comparing with Aβ group,the mRNA and protein expression of Nrf2,HO-1,GCLC and MnSOD significantly increased in Gen-treated group and Gen group(P<0.05).Conclusion Genistein could inhibit the oxidative damage induced by Aβ25-35 in astrocytes through regulating Nrf2/ARE pathway related factors.更多还原