【摘要】 目的:探讨葛根芩连汤整方剂量变化治疗2型糖尿病的量效关系,获得基于药效学的经方"剂量阈"。方法:SD大鼠给予高脂饲料喂养4周后按30 mg.kg-1尾iv新鲜配制的STZ(链脲佐菌素)诱导2型糖尿病大鼠模型,正常对照组注射同体积的柠檬酸盐缓冲液。实验分组:正常组、糖尿病模型组、葛根芩连汤整方剂量变化的9个剂量组(分别为生药1.65,4.95,8.25,11.55,14.85,18.15,21.45,24.75,28.05 g.kg-1,)、阳性药物对照组(二甲双胍200 mg.kg-1),取血检测血糖、胰岛素水平、胰岛素抵抗指数(IR)、糖化血清蛋白(GSP)、糖化血红蛋白(HbA1c)等指标。结果:给药10周后,葛根芩连汤明显降低2型糖尿病大鼠血清中的血糖水平与IR(P<0.05,P<0.01)、降低糖化血清蛋白、糖化血红蛋白水平(P<0.05,P<0.01),指标与葛根芩连汤剂量存在着一定的量效关系,在一定剂量范围内,随着葛根芩连汤整方剂量的增大,药效增强,其"剂量阈"为27.01～1.09 g.kg-1。结论:葛根芩连汤整方剂量变化治疗2型糖尿病具有良好的量-效特征。更多还原

【Abstract】 Objective: To investigate the effects of Gegen Qinlian decoction on type 2 diabetes in rats and the dose-effect relationship,to obtain the dosage threshold of the classical prescription.Method: SD rats were randomly assigned into normal control group and diabetic model group,which was established by injection small dose of streptozotosin(STZ)(30 mg.kg-1) after feeding a diet with high glucose and lipids for 4 weeks.Diabetic rats were randomly assigned into model group,dimethylbiguanide group(200 mg.kg-1) and different dose of Gegen Qinlian decoction groups(1.65,4.95,8.25,11.55,14.85,18.15,21.45,24.75,28.05 g.kg-1 accordingly).The serum glucose,insulin,insulin resistance(IR),glycosylated hemoglobin(HbA1c),glycosylated serum protein(GSP) were determined.Result: Compared with model group,Gegen Qinlian decoction could decrease serum glucose,insulin,IR,HbA1c and GSP level(P < 0.05,P < 0.01).As dose of Gegen Qinlian decoction increased,the drug action was more obviously in the specified dose range.The dosage threshold of Gegen Qinlian decoction on diabetes in rats was 27.01-1.09 g.kg-1.Conclusion: The effect of Gegen Qinlian decoction on type 2 diabetes in rats shows fine dose-effect relationship.更多还原