【摘要】 目的:观察六味地黄丸对老年大鼠学习记忆及脑内M1胆碱受体(MI-AchR)阳性神经元的影响,探讨滋阴补肾法改善老年大鼠学习记忆能力的可能机制。方法:将雄性SD大鼠分为青年对照组(3月龄)、老年对照组(24月龄)和六味地黄丸组(24月龄)。六味地黄丸组大鼠按3 g.kg-1ig六味地黄丸液,老年对照组ig同体积生理盐水,各30 d;青年对照组ig同体积生理盐水7 d。通过Morris水迷宫实验,比较其学习记忆能力,并运用免疫组织化学方法,检测大脑海马和皮质相关区域M1-AchR阳性神经元变化。结果:老年对照组各指标与青年对照组比,有极显著性差异(P<0.01)。六味地黄丸组平均逃避潜伏期自第3天起较老年对照组明显缩短,有极显著性差异(P<0.01)。目标象限游程占全部游程百分比方面,与老年对照组相比,六味地黄丸组有极显著性差异(P<0.01);六味地黄丸组与老年对照组穿越平台次数相比,有极显著性差异(P<0.01)。与老年对照组相比,六味地黄丸组M1-AchR阳性细胞数目增多,在海马CA3区及第一躯体感觉区(S1Tr)内,有极显著性差异(P<0.01)。结论:六味地黄丸可以改善自然衰老大鼠的空间学习记忆能力,可能与其保护海马CA3区及S1Tr内胆碱能毒蕈碱M1-AchR阳性神经元,增强中枢胆碱能系统功能有关。更多还原

【Abstract】 Objective:To study the effect of Liuwei Dihuang Wan on aged rats with the learning memory and cholinergic M1 receptor(M1-AchR) positive neurons.Method: The SD rats were divided into three groups: healthy young control group﹙3 month-old﹚,the natural aging model group ﹙24 month-old﹚and the Liuwei Dihuang Wan group﹙24 month-old﹚.Liuwei Dihuang Wan group and the natural aging model group were orally administered with Liuwei Dihuang Wan﹙3 g·kg-1﹚and the same volume of distilled water for thirty days;the healthy young control group was administered with the same volume of distilled water for seven days.It tested the learning and memory capacity with Morris water maze.It detected M1-AchR with immunohistochemistry.Result:All of the indexes between rats in older group and the healthy young control group were significant(P<0.01).The average escape latency of the elderly was significantly shorter than the Liuwei Dihuang Wan group since the third day,and there was a significant difference(P<0.01).Space exploration experiments showed that there was a significant difference between rats in older group and Liuwei Dihuang Wan group in the number of crossing the platform(P<0.01).Compared with the aged rats in control group,the numbers of M1-AchR positive cell of aged rats in Liuwei Dihuang Wan group increased in the hippocampus CA3 and primary somatosensory cortex;trunk region(S1Tr),and there were very significant between them(P<0.01).Conclusion: Liuwei Dihuang Wan can improve the learning and memory of the natural aging rats,which was caused possibly by protecting M1 AchR positive neurons in the hippocampal CA3 and S1Tr and enhanced the central cholinergic system function.更多还原