【摘要】 目的:建立麸炒枳壳配方颗粒的HPLC指纹图谱,为该配方颗粒的质量评价提供实验依据。方法:加甲醇超声处理制样,采用HPLC法,以Hypersil C18柱(1.6 mm×200 mm,5μm)为色谱柱,以甲醇(A)-0.1%冰醋酸(B)为流动相,进行梯度洗脱,流速1.0 mL.min-1,检测波长320 nm,柱温30℃。结果:10批麸炒枳壳配方颗粒检出8个共有峰,指认了3个特征峰。以柚皮苷为参照峰,不同批次麸炒枳壳配方颗粒相似度在0.9以上。结论:该方法准确可靠,重复性好,可为麸炒枳壳配方颗粒质量控制提供参考。更多还原

【Abstract】 Objective:To establish an HPLC fingerprint of Aurantii Fructus Praeparatus dispensing granules and provide experimental evidence for its quality criteria.Method: Take methanol extract of Aurantii Fructus Praeparatus dispensing granules as sample by ultrasonic processing,HPLC analysis was carried out on Hypersil C18(1.6 mm×200 mm,5 μm) chromatographic column with mobile phase of methanol(A)-0.1% glacial acetic acid(B),gradient elution with a flow at 1.0 mL · min-1,an ultraviolet detection wavelength at 320 nm and column temperature at 30 ℃.Result: Eight common peaks and three characteristic peaks were identified in chromatograms from ten batches of samples.The similarity is above 0.9 in different batches of samples with the peak of naringin as reference.Conclusion: The method is accurate,credible with good repeatability;it can provide reference evidence for quality control of Aurantii Fructus Praeparatus dispensing granules.更多还原