【摘要】 建立了一种柱前衍生反相高效液相色谱检测溶液中低浓度5-氨基乙酰丙酸的方法,填补了低浓度ALA的HPLC法检测的空白。使用Agilent SB-C18型色谱柱(250mm×4.6mmi.d.,5μm),以50mM甲醇-醋酸钠缓冲溶液(pH4.0)洗脱,流速为1mL/min,柱温30℃,254nm波长下检测10min。在此条件下,5-氨基乙酰丙酸在浓度为1~1000mg/L范围内与色谱峰面积值之间线性关系良好,回归系数均在0.9999以上。发酵液产物的加标回收率平均值为101.7%。将检测结果与分光光度法相比,符合较好。测试数据表明,此方法简单、可靠,对于低浓度的ALA测定效果良好。更多还原

【Abstract】 A reversed phase high performance liquid chromatography with precolumn derivation was developed for the determination of low concentration 5-aminolevulinic acid (ALA), filled the blank of low concentration of ALA detected by HPLC. An Agilent SB-C18 column (250 mm ×4.6 mm i.d., 5 μm) was used for separation, and the elution was performed with methanol and 50 mmol/L sodium acetate solution (pH 4.0). The flow rate was 1 mL/min, the temperature of column was 30℃, and the detection wavelength was at 254 nm with the detection time of 10 minutes. The dynamic linear range was 1-1000 mg/L, and the correlation coefficient was above 0.9999. The average recoveries of ALA in 3 samples of fermentation were 101.7%. The results agreed well with those of spectrophotometry. So the developed method is simple and reliable for the determination of low concentration ALA.更多还原