【摘要】 β-葡聚糖酶是重要的工业用酶,对β-葡聚糖具有水解作用,能使其降解为低分子量片段,对酿造和饲料工业有重要意义。目前,国内对β-葡聚糖酶的研究还处于初级阶段,主要集中在构建基因工程菌株来提高酶的产量和性能方面,天然菌株的产酶量及酶的性能满足不了实际应用。结合近年来相关研究,从菌种选育、培养基优化以及发酵生产三个方面,总结介绍了重组大肠杆菌高效表达细胞外β-葡聚糖酶研究进展。更多还原

【Abstract】 β-glucanase is a kind of important industrial enzyme,which can hydrolyze β-glucan to low molecular weight fragments.It has an important significance in brewing and feed industry.The domestic study about β-glucanase was in early stage,which mainly concentrates on constructing a recombinant strain and enhancing the production.The production and enzyme activity of wild strains can not meet the need of actual production yet.Relevant researches were summarized in three aspects: strain breeding,medium optimizing and fermentation,to introduce recently research progress about overexpression of extracellular β-glucanase by recombinant Escherichia coli.更多还原