【摘要】 目的:探讨内毒素脂多糖(Lipopolysaccharide,LPS)对大鼠星形胶质细胞热休克蛋白A12B(Heat shock proteins A 12B,HSPA12B)表达变化和细胞定位的影响。方法:用不同浓度(0.01、0.1、1、10μg/ml)和同一浓度(1μg/ml)不同时间(1、2、4、6、8、12、24小时)的LPS刺激星形胶质细胞,分别用RT-PCR和Western blot法检测星形胶质细胞HSPA12B的mRNA和蛋白质水平表达情况,间接免疫荧光双标法检测HSPA12B在星形胶质细胞中的细胞定位。结果:用LPS 1μg/ml刺激1小时后,HSPA12B mRNA的表达增加,4小时表达活性最高。HSPA12B蛋白水平表达变化与mRNA水平变化相一致。免疫细胞化学证明LPS诱导星形胶质细胞HSPA12B的表达定位在胞核。结论:LPS可诱导星形胶质细胞HSPA12B表达上调。HSPA12B可作为一种炎症反应蛋白参与炎症反应过程。更多还原

【Abstract】 Objective: To explore the effect of lipopolysaccharide( LPS) on the changes of the expression and intracellular location of Heat shock proteins A 12B( HSPA12B) in rat astrocytes. Methods: Astrocytes were treated with LPS at different concentration( 0. 01,0. 1,1,10 μg / ml) and at the same concentration( 1 μg / ml) for different time( 1,2,4,6,8,12,24 h). With RTPCR and Western blot measurement of HSPA12B,the levels of HSPA12B gene and protein expression were observed. Intracellular location of HSPA12B was detected under fluorescence microscope. Results: HSPA12B mRNA increased after 1 h exposure to LPS( 1μg/ml),and peaked at 4 h. And the HSPA12B protein expression was paralleled with the levels of mRNA. Immunocytochemical staining indicated the expression of HSPA12B was located in the nuclear following LPS challenge. Conclusion: Bacterial causative agent,LPS could induce the expression of HSPA12B mRNA in rat astrocytes at transcription and protein level. HSPA12B served as an inflammatory response molecule participating in the inflammatory process.更多还原