【摘要】 目的:探讨肝特异性的miR-122表达在肝癌中的功能。方法:将miR-122类似物及反义序列分别转染HepG2细胞,Real time PCR检测成熟miR-122的表达量,MTT法检测miR-122对细胞增殖的影响,DAPI染色和流式细胞技术分析miR-122诱导的细胞凋亡情况。结果:与对照组相比,miR-122转染后表达显著上调(P<0.01),明显抑制肝癌细胞的增殖,并且诱导促进肝癌细胞凋亡(P<0.01)。结论:miR-122参与对肝癌细胞的生长增殖的调控,有希望成为一个肝癌诊断及治疗的新靶标。更多还原

【Abstract】 Objective:To discuss the functional characterization of the human liver-specific miR-122 in hepatocullar carcinoma.Methods: miR-122 mimics and anti-miR-122 were transfected into HepG2 cells respectively.The expression of miR-122 was then observed by Real time PCR method,and cell proliferation was analyzed by MTT method and apoptosis was analyzed by flow cytometry.Results: miR-122 was highly expressed in miR-122 infected HepG2 cells than in controls(P<0.01)and it significantly suppressed cell growth as well as induction of apoptosis in HCC cell lines(P<0.01).Conclusion: Our results suggest that miR-122 may be a promising molecular target for HCC diagnosis and therapy.更多还原