【摘要】 为了提高酶解豆粕在水产饲料中的加工质量,以寡肽得率为指标,在单因素试验的基础上采用正交试验优化Alcalase(碱性蛋白酶)和Flavourzyme(风味蛋白酶)同步酶解工艺条件。综合考虑成本和工艺要求等因素,确定双酶法制备寡肽豆粕的最优酶解工艺条件为:底物浓度80 g/L、酶活比(Alcalase:Fla-vourzyme)2∶1、加酶量3 000 U/g、反应pH值8.0、反应温度61℃、酶解时间7.5 h。最终产物寡肽得率为38.16%,DH值为22.69%,产品与!-淀粉具有较佳的亲和性,溶失率为(2.5±0.7)%。结果表明,以寡肽得率为指标,可以有效解决豆粕蛋白质水解进程中蛋白水解过度或不足问题,制备的寡肽豆粕其黏弹性和溶失率能够满足特种水产饲料加工需求。更多还原

【Abstract】 In order to improve the aquatic feed processing quality of hydrolyzed soybean meal,single factor experiment and orthogonal test design were used for optimization of the bi-enzymatic hydrolysis conditions to produce aquatic feed oligo-peptide soybean meal in this study.Based on the evaluation of the production of soybean oligo-peptide,the optimum hydrolysis conditions were designated as substrate concentration of 80 g/L,the ratio of the two enzyme(Alcalase: Flavourzyme) of 2∶ 1,protease amount of 3 000 U/g,hydrolysis temperature of 61 ℃,pH 8.0 and reaction time of 7.5 h.Finally,a yield of soybean oligo-peptide of 38.16% and a DH of 22.69% were obtained.The resultant oligopeptide soybean product had a better processing quality with a greater affinity with-starch and a less leaching loss of product of(2.5±0.7)%.The viscoelasticity and leaching losses of oligo-peptide product were able to meet the processing requirement of aquatic feeds.The results showed that the yield of oligo-peptide was a better indication for controlling the process of soybean meal hydrolysis than the DH(degree of hydrolysis) to produce a high-quality aquatic feed oligo-peptide soybean meal.更多还原