【摘要】 目的观察转化生长因子β1(TGF-β1)、血管内皮生长因子(VEGF)在富血小板血浆(PRP)治疗大鼠跟腱断裂模型中表达水平的差异,进一步探讨PRP对跟腱断裂愈合的影响。方法选择SD大鼠46只,分为3组:空白组、PRP组、贫血小板血浆(PPP)组,每组12只,剩余10只用于PRP的制备。每组大鼠均行双后肢跟腱横段术,术后给予PRP、PPP及空白干预。术后第1、2、3、4周每组分别处死3只大鼠对跟腱行HE染色和免疫组织化学染色检查。结果在跟腱愈合过程前2周中,VEGF、TGF-β1的表达在PRP组中显著高于PPP组和空白组(P<0.05),第3、4周两者的表达在PRP组下降,空白组及PPP组第3、4周两者表达未见明显下降;术后1～4周,PRP组TGF-β1的表达与PPP组、空白组比较差异具有统计学意义(P<0.05);术后1～3周,PRP组VEGF的表达与PPP组、空白组比较差异具有统计学意义(P<0.05),第4周差异无统计学意义(P>0.05)。术后1～4周PPP组与空白组比较无统计学差异(P>0.05)。结论 PRP可以促进VEGF、TGF-β1的提前表达加速跟腱的愈合过程。更多还原

【Abstract】 Objective To observe the expression of transforming growth factor β1(TGF-β1)and vascular endothelial growth factor(VEGF)in an Achilles tendon section model after application of platelet-rich plasma for investigation of the molecular mechanisms,and further explore the influences of PRP in Achilles tendon healing.Methods Forty-six health Sprague Dawley rats,weighing 150-250 g and male or female,were randomly divided into 3 groups(control group,PRP group,PPP group),12 rats for each group.PRP was prepared form the rest of the ten rats.rats were established tendon injury models by cutting the Achilles tendon.A volume of 100 μl of PRP was then injected into the tendon mass in the PRP group once a week,100 μl PPP was injected in the PPP group,and nothing in the control group.Equal numbers of animals were sacrificed at 1st,2nd,3rd,and 4th week after operation for histological and immunohistochemical observations.Results Immunohistochemical staining showed the expression of VEGF and TGF-β1in PRP group was up-regulated at 1 week and 2 weeks,and subsequently down-regulated at 3 and 4 weeks,but in PPP and control group didnit decrease significantly,the expression of TGF-β1 showed significant differences between PRP groups and the rest of the two groups at each time point(P<0.05);the expression of VEGF showing significant differences between PRP groups and the rest of the two groups at 1 week to 3 week,but not at 4 week(P<0.05).There were no differences between the PPP group and the control group at each time point(P>0.05).Conclusions Our research suggests that the PRP may facilitate the tendon healing process,which may be associated with its altering the expression of VEGF and TGF-β1.更多还原