【摘要】 目的通过对北京市海淀区日常食品及食物中毒样本检出的金黄色葡萄球菌进行肠毒素分型检测,比较两种分离菌株的肠毒素分布差异。方法实验所用的金黄色葡萄球菌为2007-2012年海淀区日常食品和食物中毒样本中分离检出的菌株,依据GB 4789.10-2010采用ELISA方法测定金黄色葡萄球菌肠毒素(SEA-SEE)。结果 127株金黄色葡萄球菌中有108株肠毒素阳性,产肠毒素阳性率为85.0%。日常食品检出金黄色葡萄球菌93株,76株菌产肠毒素,产肠毒素阳性率为81.7%;食物中毒样本检出金黄色葡萄球菌34株,32株菌产肠毒素,产肠毒素阳性率为94.1%。产1种肠毒素和同时产3种肠毒素的菌株分别是47、37株,在产毒株中分别占43.5%和34.3%。食物中毒分离株产SEA和SED的比例(65.6%、65.6%)大于日常食品分离株(32.9%、30.3%).共有98株菌产SEE,在产毒株中占90.7%,肠毒素类型分布由高到低依次为E、A、D、C、B。结论食源性金黄色葡萄球菌产毒素能力较强,金黄色葡萄球菌食物中毒分离株和日常食品分离株在肠毒素分布上有差异。更多还原

【Abstract】 Objective To compare the distribution and difference of the enterotoxin type of Staphylococcus aureus(S.aureus)isolated from food and food poisoning samples in Haidian district.Methods Bacteria strains of S.aureus were isolated from food and food poisoning samples in Haidian district of Beijing from 2007 to 2012.The type of Enterotoxin was determined based on GB 4789.10-2010,A-E staphylococcal enterotoxinproducting assay by ELISA(SEA-SEE).Results Enterotoxin was found in 108out of 127strains of S.aureus with the overall positive rate of 85%,including 76strains from 93S.aureus isolated from food samples(81.7%)and 32strains from 34S.aureus isolated from food poisoning samples(94.1%).There were 47strains(43.5%)in which only one type of enterotoxin was identified,while 37strains(34.3%)were found to be able to produce 3types of enterotoxins.Enterotoxin SEA and SED were mainly found in strains isolated from food poisoning samples(65.6%and 65.6%).98strains were found to produce SEE,accounting for 90.7%of total enterotoxin-positive strains,which was the most common type identified in the study,followed by A,D,C and B.Conclusions Food-borne S.aureus has higher ability to produce enterotoxin and the types of enterotoxin produced by S.aureus isolated from food samples are different from those isolated from food poisoning samples.更多还原