【摘要】 目的探讨汉黄芩素对胶质瘤U251细胞SurvivinmRNA及Caspase-3 mRNA表达的影响。方法 192株人胶质瘤U251细胞株分别用汉黄芩素(96株)、替莫唑胺(TMZ)(48株)处理,留取48株作为空白对照组,半定量RT-PCR法检测Surviv inmRNA与Caspase-3 mRNA表达强度,并在3组之间进行对比分析。结果空白对照组Surviv inmRNA表达强度为(0.773±0.176)μM,显著高于汉黄芩素组的(0.382±0.087)μM及TMZ组的(0.408±0.092)μM(P<0.05);Caspase-3 mRNA表达强度为(0.097±0.043)μM,显著低于汉黄芩素组的(0.454±0.213)μM及TMZ组的(0.432±0.187)μM(P<0.05),汉黄芩组与TMZ组之间比较差异无统计学意义(P>0.05)。结论汉黄芩素可通过下调Surviv inmRNA的表达并上调Caspase-3 mRNA的表达起到有效诱导人胶质瘤U251细胞凋亡的作用。更多还原

【Abstract】 Objective To discuss the effects of wogonin on the expression of Survivin mRNA and Caspase-3 mRNA in human giloma cell line U251.Methods One hundred and ninety-two human giloma cell line U251 were cultured with wogonin(96 strains) and TMZ(48 strains),and other 48 strains were as blank control group,Survivin mRNA and Cas pase-3 mRNA expression level were detected by semi-quantitative RT-PCR,and then Survivin mRNA and Caspase3 mRNA expression level were contrasted between the three groups.Results Survivin mRNA expression level in the blank control group was(0.773 ±0.176) μM,significantly higher than(0.382 ±0.087) μM in the wogonin group and(0.408±0.092) μM in the TMZ group(P < 0.05),and Caspase-3 mRNA expression level was(0.097 ±0.043) μM,significantly lower than(0.454±0.213) μM in the wogonin group and(0.432±0.187) μM in the TMZ group(P < 0.05),but there was no significantly difference in Survivin mRNA and Caspase-3 mRNA expression level between wogonin group and TMZ group(P > 0.05).Conclusion Wogonin play a role in inducing apoptosis in glioma U251 cells through reducing the expression of Survivin mRNA and raising the expression of Caspase-3 protein expression.更多还原