乳腺浸润性导管癌临床病理因素与BRCA₁和BRCA₂表达的相关性分析

【摘要】 目的:探讨乳腺上皮中BRCA1和BRCA2的表达与乳腺浸润性导管癌的临床病理特征的相关性。方法:应用免疫组化S-P法检测BRCA1、BRCA2在33例乳腺浸润性导管癌组和31例乳腺良性病变组的表达情况,并联合ER、PR、CerBb-2的表达,分析它们与乳腺浸润性导管癌患者的年龄、肿块大小、分化程度、淋巴结转移等特征之间的关系。结果:①BRCA1的阳性表达与乳腺浸润性导管癌患者的年龄、分化程度、是否绝经均有显著相关性(P<0.05),与淋巴结转移和肿瘤大小无显著相关性(P>0.05)。BRCA2的阳性表达与乳腺浸润性导管癌患者的年龄、淋巴结转移、肿块大小、是否绝经均有显著相关性(P<0.05),与分化程度无显著相关性(P>0.05)。②BRCA1、BRCA2的表达与CerBb-2的表达均呈显著的负相关(r=-0.355,-0.517,P<0.05)。结论:BRCA1和BRCA2的突变在乳腺浸润性导管癌的发生发展密切相关,主要通过转录途径作用于CerBb-2,BRCA1和BRCA联合CerBb-2可作为评价乳腺癌生物学特性和预后的潜在指标。更多还原

【Abstract】 Objective:The expressions of breast cancer susceptibility gene1(BRCA1)and breast cancer susceptibility gene2(BRCA2)in breast epithelia are explored. The intrinsic relevance among the expressions of BRCA1 and BRCA2,the development and prognosis in invasive ductal carcinoma of breast are investigated.Methods:The immunohistochemical S-P method was used to detect the level of BRCA1,BRCA2 in 33 cases of breast invasive ductal carcinoma tissue samples and 31 cases of breast benign disease tissue samples.Associating with the expressions of estrogen receptor(ER),progestogen receptor(PR),and epidermal growth factor receptor2(CerBb-2),an analysis is needed in the relationship between the expressions of ER,PR,CerBb-2,BRCA1,BRCA2 and the characters of patients such as the age,the size of tumor,the grade of differentiation,and lymph node metastasis.Results:①The positive expression in BRCA1 exhibits an apparent relevance with the characters of patients such as the age,the size of tumor,the grade of differentiation,lymph node metastasis,menopausal age(P<0.05),while it shows little correlations to the grade of differentiation (P>0.05).②The expressions of BRCA1 and BRCA2 were negatively correlated to the expression of CerBb-2(r=-0.355,or -0.517,P<0.05).Conclusion:The mutation of BRCA1 and BRCA2 takes a key role in the generation of breast invasive ductal carcinoma,which can be as a reference to detect biological behavior or prognosis in breast carcinoma.更多还原