【摘要】 为了提高红麻愈伤组织不定芽诱导率,以红麻子叶和胚轴为外植体,采用两种途径诱导红麻不定芽分化,对影响红麻不定芽分化的培养基组成因素进行研究。结果表明:(1)在诱导愈伤组织分化不定芽的途径中,两种外植体均可诱导产生愈伤组织,但是胚轴产生的愈伤组织质量优于子叶。在分化培养基MS+6-BA(1mg/L)+2,4-D(1mg/L)+GA3(1mg/L)中,愈伤组织可分化少量不定芽,但是分化率较低;当该分化培养基中添加0.3%的活性炭时,愈伤组织在分化不定芽的同时也产生了大量不定根。(2)在通过直接诱导外植体分化不定芽的途径中,胚轴难以诱导产生不定芽,子叶可有效诱导不定芽;在MS+TDZ(0.1mg/L)+NAA(0.1mg/L)的诱导培养基中,子叶的不定芽诱导效果最好,诱导率为64.29%。而在诱导培养基中添加AgNO3后,不定芽的诱导率明显提高,其诱导效果最好的培养基为MS+TDZ(0.1mg/L)+NAA(0.1mg/L)+AgNO3(1mg/L),此时不定芽诱导率可达85%。更多还原

【Abstract】 Cotyledon and hypocotyl were used as explants to study the influential factors of kenaf adventitious buds differentiation through two approaches.The results showed that:(1) in the approach of callus induction,the quality of hypocotyl callus was better than cotyledon,callus could be induced to differentiate a few adventitious buds in the medium of MS + 6-BA (1mg/L) + 2,4-D (1mg/L) +GA3 (1mg/L),but the induction rate was very low.After adding 0.3% activated carbon in the medium,many roots were induced along with adventitious buds.(2) In the approach of inducing explants directly,hypocotyl hardly generated adventitious buds,cotyledon could induce adventitious effectively.Cotyledon could get the highest induction rate in the medium of MS + TDZ (0.1mg/L) + NAA (0.1mg/L),and the rate was 64.29%.After adding AgNO3 in the medium,the rate could increase obviously.The best induction medium was MS + TDZ (0.1mg/L) + NAA (0.1mg/L) + AgNO3 (1mg/L),and the adventitious buds induction rate was 85%.更多还原