【摘要】 目的探讨沙樱桃脂类对人胃癌MGC-803细胞的毒性作用及其机制。方法采用台盼蓝法检测细胞数、流式细胞仪检测细胞凋亡。将人MGC-803细胞分别与不同浓度的沙樱桃脂类(沙樱桃脂组)、5-FU(5-FU组)及PBS溶液(对照组)共同培养,观察沙樱桃脂类对人胃癌MGC-803细胞增殖的抑制作用。结果对人MGC-803细胞分别给予0.5、1.0、3.0mg/L沙樱桃脂类及10mg/L5-FU,培养6h、12h、18h、24h和48h,活细胞数随沙樱桃脂类浓度的增加和作用时间的延长而减少,与5-FU组相似(P>0.05),而与对照组比,差异有统计学意义(P<0.01);分析存活细胞率,表明人MGC-803细胞增殖被阻滞于G0G1期。在给予沙樱桃脂类0.5、1.0、3.0mg/L和5-FU培养48h后,人MGC-803细胞的凋亡率分别为13.8%、19.6%、26.2%和21.1%。结论沙樱桃脂类对人胃癌MGC-803细胞具有显著毒性作用,其机制可能与沙樱桃脂类可抑制MGC-803细胞分裂,并诱导其凋亡有关。更多还原

【Abstract】 Objective To investigate toxic effect of shayingtao lipids on human gastric carcinoma MGC-803 cells proliferation and its potential mechanism.Methods The living cell were determined by trypan blue method,and the cell apoptosis was detected by flow cytometry.The MGC-803 cells were cultured together with shayingtao lipids of different concentration (shayingtao lipids group),5-FU (5-FU group) and PBS solution (control group) respectively.The inhibition effects of shayingtao lipids and 5-FU on MGC-803 cell proliferation were observed and compared.Results Culturing MGC-803 cells together with 0.5,1.0,3.0 mg / L shayingtao lipids and 10mg/L 5FU for 6h,12h,18h,24h and 48h,the number of living cells decreased along with the increase of shayingtao lipids concentration and prolongation of action time,and the result was similar with 5-FU(P>0.05)while had statistical difference compared with control group (P<0.01).According to analysis of cell living rate,the proliferation of MGC-803 cells was blocked in G0G1 stage.Culturing MGC-803 cells together with 0.5,1.0,3.0 mg/L shayingtao lipids and 10 mg / L 5-FU for 48h,the apoptosis rates of MGC-803 cell were 13.8%,19.6%,26.2% and 21.1% respectively.Conclusion Shayingtao lipids have significant toxic effect on human gastric carcinoma MGC-803 cells,and the mechanism might be implicated in inhibiting cell division and inducing cell apoptosis.更多还原