【摘要】 背景与目的:在乳腺癌微环境中,肿瘤相关巨噬细胞(tumor associated macrophages,TAMs)分泌CCL18,CCL18与病人的预后呈负相关。本文旨在探讨来源于TAMs的CCL18是否能促进乳腺癌细胞黏附于细胞外基质(extracellular matrix,ECM),进一步阐明CCL18促进乳腺癌细胞黏附于ECM的分子机制。方法:在无血清培养体系中,用纤粘连蛋白包被培养板,用黏附实验的方法检测CCL18对乳腺癌SK-3rd细胞黏附于ECM的作用;用免疫荧光的方法检测CCL18对SK-3rd细胞表面整合素的影响。结果:CCL18通过引起乳腺癌SK-3rd细胞表面整合素的聚集,导致乳腺癌SK-3rd细胞黏附于ECM的数量明显增多。结论:CCL18通过促进整合素聚集而促进乳腺癌细胞黏附于ECM。更多还原

【Abstract】 Background and purpose:In the microenvironment of breast cancer,tumor associated macrophages(TAMs) secrete CCL18,and CCL18 has negative correlation with prognosis of patients.The present study investigated whether CCL18 derived from TAMs,promoted breast cancer cells adherence on extracellular matrix(ECM),and further interpreted the molecular mechanism of CCL18 promoting breast cancer adherence.Methods:In the serum-free culture system of breast cancer cells,in cultivation plate coated with fibronectin,adherence assay was performed to detect CCL18 function on breast cancer SK-3rd cells to adherence on ECM,and immunofluorescence was carried out to detect CCL18 influence integrin aggregation on SK-3rd.Results:CCL18 elevated the number of SK-3rd breast cancer cells to adherence on ECM,via integrin α5β1 aggregation.Conclusion:CCL18 promotes breast cancer cells adherence on ECM via integrin aggregation.更多还原