【摘要】 目的:确定人单纯疱疹病毒2(HSV2)和衣原体DNA在标本宫颈腺瘤中是否出现,以及与HPV感染之间的相关性。方法:从257份宫颈腺瘤石蜡包埋标本提取DNA,采用PCR法对DNA进行扩增,并进行HPV基因分型,检测CT和HSV-2 DNA的阳性率。结果:257份石蜡包埋标本中,67份标本能检出β-globin DNA序列,其中HPV DNA阳性率为79.4%,其中HPV18和HPV16分别为51%和34%。在所有这些标本中没有检测到HSV2和衣原体DNA。结论:没有检测到HSV2和衣原体DNA整合到宫颈腺癌的细胞基因组,也没有发现它们是HPV所致的宫颈腺癌的辅助致病因子。更多还原

【Abstract】 Objective: To explore the appearance situation of Herpes simplex virus type 2( HSV-2) and Chlamydia DNA in cervical adenoma specimens and their correlations with human papillomavirus( HPV) infection. Methods: DNA was abstracted from 257 paraffin- embedded cervical adenoma specimens,PCR was used to amplify DNA,HPV genetic typing was conducted,the positive rates of Chlamydia trachomatis( CT) and HSV- 2 DNA were detected. Results: A total of 257 specimens were detected,and β- globin DNA sequence was found in 67 specimens,the positive rate of HPV DNA was 79. 4%,the positive rates of HPV18 and HPV16 were 51% and 34%,respectively. HSV2 and CT DNA were not found in all the specimens. Conclusion: In the study,HSV- 2 DNA and CT DNA were not found to be integrated to the genome of adenocarcinoma of the uterine cervix and they do not seem to be a co- factor for HPV on the etiology of adenocarcinoma of uterine cervix.更多还原