【摘要】 目的:研究抗环瓜氨酸肽(anti-cyclic citrullinated peptide,CCP)抗体与类风湿关节炎(rheumatoid arthritis,RA)患者成纤维样滑膜细胞(fibroblast-like synoviocyte,FLS)迁移和侵袭的关系。方法:FLS分离自22例活动性RA患者、12例骨关节炎(OA)和6例无关节炎病史的外伤患者滑膜组织;FLS迁移和侵袭能力采用Transwell小室测定。结果:RA组、OA组和正常组FLS迁移数目分别为(29.33±10.93)、(9.28±7.87)和(7.00±4.07)个/视野,侵袭细胞数分别为(14.35±7.67)、(3.96±4.37)和(3.08±1.03)个/视野,RA组与其它2组的迁移细胞数和侵袭细胞数比较均有显著差异;抗CCP抗体阳性RA患者FLS与抗CCP阴性RA患者FLS相比,前者迁移和侵袭能力均强于后者;相关性分析亦发现,抗CCP抗体阳性及抗CCP抗体滴度与RA患者FLS的侵袭和迁移能力密切相关。结论:抗CCP抗体阳性的RA患者FLS的迁移和侵袭能力更强,抗CCP抗体可能与RA患者FLS的迁移和侵袭能力改变有关。更多还原

【Abstract】 AIM: To investigate the relationship between anti-cyclic citrullinated peptide( anti-CCP) antibody and migration or invasion ability of fibroblast-like synoviocytes( FLS) from rheumatoid arthritis( RA) patients. METHODS: FLS was isolated from 22 patients with active RA,12 patients with osteoarthritis( OA) and 6 patients with joint injuries without arthritis history. The migration and invasion abilities were assessed by Transwell assay. RESULTS: Among the patients of RA,OA and normal controls,the numbers of migratory cells were( 29. 33 ± 10. 93),( 9. 28 ± 7. 87) and( 7. 00 ±4. 07) /field,respectively,and the numbers of invasive cells were( 14. 35 ±7. 67),( 3. 96 ±4. 37) and( 3.08 ± 1. 03) / field,respectively. The numbers of migratory and invasive cells were higher in RA than those in the other groups. The migration and invasion abilities of RA FLS from anti-CCP antibody-positive RA patients were significantly increased as compared with anti-CCP antibody-negative RA patients. Correlation analysis revealed that the migration and invasion abilities of RA FLS were correlated with the positive and high titer of anti-CCP antibody. CONCLUSION: The migration and invasion abilities of FLS from anti-CCP antibody-positive RA patients are increased. Anti-CCP antibody is possibly correlated with the increased migration and invasion abilities of RA FLS.更多还原