【摘要】 本研究通过检测RAGE-1基因在急性髓系白血病(AML)患者骨髓单个核细胞(BMMNC)中的表达,探讨其表达水平与临床变量的相关性。应用RQ-PCR方法检测94例初诊AML患者BMMNC中RAGE-1基因表达水平,分析其与患者年龄、性别、血象、诊断分型和预后的关系,并比较治疗前后RAGE-1表达量的变化。结果表明:26例(28%)AML患者存在着RAGE-1转录本的过表达(1.34-16.34,中位3.07);RAGE-1过表达阳性组与阴性组在性别、年龄、血象和FAB亚型之间均无显著性差异;不同核型预后分组之间RAGE-1过表达频率无显著性差异,且不同类型核型AML患者中RAGE-1转录本过表达频率也无差异。治疗后获得完全缓解的患者RAGE-1表达水平较治疗前明显降低。RAGE-1过表达组与阴性组患者的总体生存时间并无显著性差异。结论:RAGE-1基因过表达是AML中的一个常见分子事件,但对患者预后并无明显影响。更多还原

【Abstract】 The purpose of this study was to detect the expression of RAGE-1 transcript in the bone marrow mononuclear cells(BMMNC) from patients with acute myeloid leukemia(AML) and to investigate the relationship of RAGE-1 expression level with clinical variables.The expression level of RAGE-1 gene in BMMNC from 94 newly diagnosed AML patients was measured using RQ-PCR.The relationship between RAGE-1 expression level and clinical parameters(age,sex,blood cell counts,diagnosis and prognosis) was investigated,and the levels of RAGE-1 expression were compared in patients before and after treatment.The results showed that overexpression of RAGE-1 transcript was found in 28%(26/94) AML patients(1.34-16.34,median 3.07).No significant difference was observed in sex,age,blood parameters and FAB subtypes between the groups with and without RAGE-1 overexpression.There was also no significant difference in the frequency of RAGE-1 overexpression among different cytogenetic risk groups and among the patients with different types of karyotypes.The level of RAGE-1 transcript significantly decreased in those patients obtained complete remission after treatment.The overall survival of AML patients with RAGE-1 overexpression was similar as that in those without RAGE-1 overexpression.It is concluded that RAGE-1 overexpression is a common event in AML,but has no impact on the prognosis of patients.更多还原