【摘要】 应用全基因组DNA甲基化芯片(Illumina Infinium HumanMethylation27 BeadChip)杂交技术以及转录组RNA测序技术,检测了雄激素依赖性前列腺癌(androgen-dependent prostate cancer,ADPC)细胞株LNCaP和雄激素非依赖性前列腺癌(androgen-independent prostate cancer,AIPC)细胞株LNCaP-AI(androgen independent)中的差异甲基化基因。发现与LNCaP细胞株相比,LNCaP-AI细胞株有990个CpG位点表现为高甲基化,涉及855个基因;2 305个CpG位点表现为低甲基化,涉及1 970个基因。结合转录组mRNA表达结果,筛选出6个超甲基化基因:FAM111B、RAB36、PCDH7、COL6A2、IGF1R、GULP1;8个低甲基化基因:EPHA3、TM4SF1、IGFBP5、FAM105A、RASD1、ITPR2、CYP27B1、UBE2E3。这些差异甲基化基因涉及钙离子信号通路、Wnt信号通路等多个信号通路,参与了细胞的基因表达、信号传导、细胞通讯、细胞运动、细胞黏附以及血管生成等功能,为探讨这些差异甲基化基因在前列腺癌雄激素非依赖性转化过程中发挥的作用奠定了基础。更多还原

【Abstract】 An integrated analysis of Illumina Infinium HumanMethylation27 BeadChip platform and transcriptome RNA sequencing technology was performed to screen out the differential methylated genes between androgen-dependent prostate cancer cell lines and androgen-independent prostate cancer cell lines.Compared with LNCaP,990 hypermethylated CpG sites(involved 855 genes) and 2 305 hypomethylated CpG sites(involved 1 970 genes) were identified in LNCaP-AI.Combined with transcriptome sequencing data,some hypermethylated genes(including FAM111B,RAB36,PCDH7,COL6A2,IGF1R and GULP1) and hypomethylated genes(including EPHA3,TM4SF1,IGFBP5,FAM105A,RASD1,ITPR2,CYP27B1 and UBE2E3) were screened out.These differential methylated genes were thought to be involved in some signaling pathways,such as the calcium signaling pathway and the Wnt signaling pathway,participating in cell biological process and its regulation,gene expression,signal transduction,cell communication,cell movement,cell adhesion,angiogenesis and so on.Our data provide a foundation for future study on the differential methylated genes in prostate cancer androgen-independent conversion process.更多还原