【摘要】 建立了维生素B2片剂中维生素B2含量的核磁共振测定方法。核磁共振的测定条件:脉冲程序为cpmgpr1d,氢谱宽度为6 009 Hz,脉冲宽度为11.95μs,延迟时间为6.50μs,采样温度为27℃,采样次数为32次。以参比物质3-(三甲基硅基)氘代丙酸钠(TSP)为内标,将不同浓度标准品与内标的NMR峰面积比对维生素B2浓度绘制标准曲线,结果显示,维生素B2在1.25~10 mg/mL范围内呈良好线性,其线性方程为:y=2.989 4 x+0.185 5,r2=0.999 2。方法的加标回收率为83.2%。该方法灵敏度高、重现性好、操作简便,可用于维生素B2片剂中维生素B2含量的测定。更多还原

【Abstract】 A NMR method was developed for the determination of vitamin B2( VB2) in vitamin B2 tablets. NMR determination conditions were as follows: the pulse sequence: cpmgpr1d,hydrogen spectral width: 6 009 Hz,pulse width: 11. 95 μs,delay time: 6. 50 μs,temperature: 27 ℃,number of scans: 32. A good linear relationship was obtained between peak area ratio of VB2 to TSP and concentrations of VB2 in the range of 1. 25- 10 mg / mL. The regression equation was y = 2. 989 4 x + 0. 185 5( r2= 0. 999 2). The average recovery was 83. 2 %. The results indicated that the NMR-based method had high sensitivity and good repeatability,and could be used in the determination of VB2 in vitamin B2 tablets.更多还原