【摘要】 基于6-丙基-2-硫脲嘧啶（PTU）对三联吡啶钌(Ru（bpy）₃²⁺)电化学发光（ECL）有强烈的增敏作用,建立了流动注射电化学发光（FI-ECL）检测PTU的新方法。通过考察影响体系电化学发光的因素(如电压信号类型及电位、缓冲溶液pH值、载液流速及Ru（bPY）₃²⁺浓度等),得到FI-ECL测定PTU的最佳条件,并提出了可能的反应机理。研究结果表明,在0.1 mol/L pH 12.0的H₃PO₄-NaOH缓冲介质中,控制恒电位为+1.50 V,Ru（bpy）₃²⁺浓度为1.0×10^-4mol/L,载液流速为1.5 mL/min,PTU对Ru（bpy）₃²⁺的电化学发光具有最大的增敏作用。在此条件下,测得PTU的线性范围为2.0×10^-71.0×10^-4mol/L（r²=0.998 6）,检出限（S/N=3）为1.3×10^-8mol/L,对7份浓度均为1.0×10^-6mol/L的PTU标准溶液进行测定,所得结果的相对标准偏差为1.2%。该方法简便、快速、灵敏,用于模拟动物饲料中PTU含量的检测,结果满意。更多还原

【Abstract】 Based on the fact that 6-propy-2-thiouracile（PTU） could strongly enhance the electrochemiluminescence（ECL） of tris（2,2’-bipyridine） ruthenium（Ⅱ）(Ru（bpy）₃²⁺),a new flow injection analysis method with a homemade electrochemiluminescence（FI-ECL） detector was proposed for the first time.The experimental parameters those may influence the ECL intensity of the Ru（bpy）₃²⁺ /PTU system(such as voltage signal mode and applied potential,pH of buffer solution,flow rate of the carrier solution,and concentration of Ru（bpy）₃²⁺ solution) were investigated.The optimum conditions for the FI-ECL detection of PTU were obtained and the possible mechanism of the ECL system was suggested.The results showed that in 0.1 mol?L^-1 pH 12.0 H,PO₄-NaOH buffer solution,when the carrier solution’s flow rate was setted at 1.5 mL?L^-1,the strongest ECL intensity enhancement could be realized at potential of + 1.50 V in the present of 1.0×10^-4 mol/L Ru（bpy）₃²⁺.Under this optimal condition,the linear range of PTU was 2.0×10^-7-1.0×10^-4 mol/L with a correlation coefficient of 0.998 6 and a detection limit（S/N=3） of 1.3×10^-8 mol/L.The relative standard deviation（RSD,n=3）for l.0×l0^-6mol/L PTU standard solution was 1.2%. The method was simple,rapid and sensitive,and was successfully applied in the determination of PTU in spiked animal feeds.更多还原