【摘要】 目的:观察大鼠在过度训练状态下骨骼肌糖代谢的变化,探讨过度训练对糖代谢的影响。方法:将SD大鼠随机分为对照组(7只,Con)、运动组(8只,Mtr)和过度训练组(9只,Otr),进行9周跑台训练,其中Otr组后3周为力竭性运动。运动后分别测定骨骼肌糖原含量,LDH、PK、SDH、SOD酶的活性及MDA浓度,电镜观察肌组织的变化。结果:Otr组肌糖原含量较Mtr组降低,各种酶活性受到不同程度的抑制,MDA浓度升高,线粒体水肿,呈空泡状。结论:大鼠连续力竭性运动所致过度训练存在骨骼肌糖代谢功能紊乱,其可能与力竭性运动引起的自由基损害有关。更多还原

【Abstract】 Objectives:It is to observe the changes in glucose metabolism of skeletal muscle in rat after overtraining and explore the effects of overtraining on glucose metabolism.Methods;SD rats were randomly divided into group Con(7,sedentary control),group Mtr(8,moderate intensity training) and group Otr(9,over training).Both training groups were doing 9-week treadmill training.Skeletal muscle glycogen levels,LDH,PK,SHD,SOD activity and MDA concentrations were determined and morphological changes of muscular tissue were observed by electron microscopy after the 9-week training.Results;Muscle glycogen levels in group Otr were lower compared with that in group Mtr.A variety of enzymatic activity was subjected to varying degrees of inhibition.MDA concentrations increased,and morphology of mitochondria was swelling to be vacuoles.Conclusions:The dysfunction of glucose metabolism in rat skeletal muscle after overtraining is probably owing to the free radical impairment from the exhaustive exercise.更多还原