【摘要】 以百里香(T.quinquecostatus)种子为材料,研究了秋水仙素不同浓度及处理时间对其种子苗多倍化变异的影响。结果表明:0.20%秋水仙素处理6h的诱变效果最佳,种子发芽率为14.00%,种子苗形态变异率为5.00%。诱导后形成的多倍化植株表现为叶片变宽变大、叶色变深、茎变粗且节距变大等形态特征。叶片显微观测表明多倍化植株的气孔显著增大,单位叶面积气孔数目减少。染色体计数表明,多倍化植株染色体多为四倍体(2n=4x=48),还有少数六倍体和非整倍体,同时存在混倍化现象。更多还原

【Abstract】 The thyme seeds were treated with colchicines at different concentration and treatment time,and the characteristics of polyploidization mutation seedling were studied.The results showed that the best treatment condition was 0.20% colchicines with 6 hours,the seed germination rate was 14.00%,morphological variation rate was 5.00%.The morphology of polyploidizing induction seedlings mainly manifested as the wider blade,darker leaf color,and thicker stem and longer stem node.Microscopic observation of polyploidization mutation seedling showed that the size of stomata increased and density of stomata decreased significantly.Chromosome counts proved that most of polyploidization mutation plants were tetraploid(2n=4x=48),with small amount of hexploid and aneuploid plants.Meanwhile,there existed the few mixoploids phenomena.更多还原