【摘要】 目的观察分泌表达NT4-TAT-6×His-VHLβ肾癌抑制融合肽的重组腺伴随病毒作用于肾癌鸡胚移植瘤的疗效。方法将人肾癌细胞系RLC-310接种到鸡胚绒毛尿囊膜上。选取成瘤较好鸡胚,分别用rAAV/NT4-TAT-6×His-VHLβ(A组)、空病毒(B组)、PBS缓冲液(C组)进行干预,观察移植瘤生长及各组移植瘤的大小,采用HE染色及免疫荧光法比较各组的作用效果。结果 A组移植瘤生长速度明显慢于其他两组;A组移植瘤体积与B、C两组比较差异有统计学意义(P<0.05);HE染色结果显示,A组鸡胚移植瘤生长受到抑制;荧光显微镜可观察到A组荧光明亮(++),对照组未观察到荧光(-)。结论 rAAV/NT4-TAT-6×His-VHLβ对肾癌鸡胚移植瘤有抗肿瘤效应,为肾癌的基因治疗研究提供了一种新的途径。更多还原

【Abstract】 Objective To study the anti-tumor effect of recombinant adeno-associated virus mediated secretive expression of NT4-TAT-6×His-VHLβ fusion peptide(rAAV/NT4-TAT-6×His-VHLβ) on the chorioallantoic membrane(CAM) implanted tumors.Methods RLC-310 cells were inoculated on the CAM.Chick embryos with implanted tumor were divided into three groups,and respectively treated with rAAV/NT4-TAT-6×His-VHLβ(group A),AAV(group B) and PBS(group C).The growth of implanted tumors were observed,and the size of tumors on fifth day after the treatment was measured.Then the effect of anti-tumor in each group was observed through HE dye and immunofluorescence.Results The results showed that the growth of implanted tumors in group A was slower than the other two groups.The tumor volumes of the group A showed significant difference compared with group B and C(P< 0.05).The growth ability of tumors was inhibited in group A.Bright fluorescent appeared in the group A(+ +).No fluorescence was found in the contrast group(-).Conclusion rAAV/NT4-TAT-6×His-VHLβ has the anti-tumor effect on chick embryo implanted tumors,which may be a novel tool for gene therapy of human renal cell carcinoma.更多还原