【摘要】 目的:研究以热水浸提法从姬菇中提取的姬菇多糖(PCNP)对酒精所致的急性损伤小鼠肝组织超微结构的影响.方法:84只小鼠被随机分为空白对照组、模型组、阳性对照组(150 mg/kg.bw)、多糖各剂量组((100、200、400)mg/kg.bw).连续灌胃30 d后,给予浓度为50%的乙醇溶液,建立动物急性肝损伤模型.12 h后,小鼠脱臼处死,取肝脏制成超薄切片,电镜观察肝组织超微结构的变化.结果:模型组细胞核的核膜不规则、呈锯齿状,线粒体畸形、嵴不清楚、糖原增多、出现空泡,内质网扩张、其膜上附着的核糖体脱失,细胞核周围脂滴数明显增多、胆管微绒毛脱失;姬菇多糖组肝细胞核膜凹凸不平,线粒体肿胀、线粒体内糖原沉积较少,内质网扩张不明显,细胞内脂滴减少.结论:姬菇多糖对酒精性肝损伤小鼠的肝细胞具有保护作用.更多还原

【Abstract】 Objective:The present study investigated the protective effect of polysaccharide from Pleurotus cornucopiae(PCNP)on electron microscopic changes in acute alcoholic hepatic injury in mice.Methods:The 84 mice were randomly divided into blank control group,alcoholic model groups.The mice in alcoholic model groups were administered by gavage with BIFENDATE(150 mg/kg·bw)or PCNP((100、200、400)mg/kg·bw)for 30 d before the administration with dose of 50% alcohol(12 mL/kg·bw).After the last alcohol treament for 12 h,in order to observe the ultrastructural changes of liver cells,all mice were dislocation death to collect samples for preparing ultrathin section for electron microscopy.Result:In model group,hepatic cells exhibited in different degrees of karyopycnosis and the nuclear membranes were irregular;The pathological changes in mitochondria had been clearly observed.In the hepatic cell,vacuoles can be seen and glycogen was increased.The rough endoplasmic reticulum were found as expansion and degranulation;Many large lipid droplets were found around the nucleus and microvilli of bile duct lost.In the PCNP groups,karyolemma were uneven.There were less expansion of mitochondria and less glycogen deposition compared with other groups;In addition,endoplasmic reticulum was not obvious outspread and lipid droplets reduced in cells.Conclusion:It can be seen that PCNP has the protective effect on hepatic ultrastructure induced by alcohol.更多还原