【摘要】 目的评估突触后致密物(PSD)95多肽疫苗对神经病理性痛大鼠的镇痛效应。方法用SD大鼠制备保留性神经损伤(SNI)模型,随机分为PSD95组、PBS组和钥孔虫戚血蓝蛋白(KLH)组。PSD95组注射3次PSD95多肽疫苗50μl,后两组分别给予PBS或KLH作为对照。分别于制备SNI前1d、第1次免疫前1d及第3次免疫后14d时测定术侧机械痛阈;并于第1次免疫前1d及第3次免疫后14d时取脑脊液、血清,测定PSD95抗体滴度;测定脊髓背角PSD95蛋白表达水平。结果 PSD95组第1次免疫前1d时未检测到PSD95抗体,而第3次免疫后14d时检测到抗体。与SNI前比较,各组机械痛阈均降低(P<0.05);PSD95组第3次免疫后机械痛阈较其余两组升高,PSD95蛋白表达下调(P<0.05)。结论 PSD95多肽疫苗对神经病理性痛大鼠具有镇痛作用,其机制可能与进入脊髓的PSD95抗体下调了PSD95有关。更多还原

【Abstract】 Objective To evaluate the analgesic effect of postsynaptic density-95 peptide as subunit vaccine against neuropathic pain in rats.Methods SD rats were randomly divided into group PSD95,group PBS and group KLH afrer spared nerve injury(SNI).The PSD95 group was immunized with subcutaneous PSD95 peptide vaccine 3 times.Group PBS and KLH served as control groups.Threshold to noxious mechanical stimulation was measured lday before SNI surgery(baseline),1 day before the 1st immunization and 14 days after the 3rd immunization.Blood and cerebral spinal fluid(CSF) samples were obtained lday before the 1st immunization and 14 days after the 3rd immunization for detection of PSD95 antibody.The expression of PSD95 protein of the lumbar segment of rat spinal cord was determined.Results PSD95 antibody was detected in CSF at 14d after the 3rd immunization in group PSD95.The threshold to mechanical stimulation was significandy decreased after SNI as compared with the baseline values in all 3 groups.The paw withdrawal threshold to mechanical stimulation after the 3rd immunization was significantly higher and the expression of PSD95 protein in die dorsal horn of the spinal cord was significantly lower in PSD95 group than in PBS and KLH group.Conclusion PSD95 peptide has analgesic effect on neuropathic pain,which could be related to the downregulated expression of PSD95.更多还原