【摘要】 [目的]建立低成本简单、而灵敏度高、准确性好的检测汞离子新方法。[方法]利用分光光度计扫描加入汞离子前后胶体金-DNA体系光谱,通过比较加入汞离子前后光密度比值(D660nm/D530nm)变化大小来快速定量汞离子。[结果]方法的线性范围为0.1～2.0μg/L,线性相关系数r=0.995,检出限为0.2μg/kg。[结论]胶体金-DNA体系法测定食品中汞离子简便、灵敏。更多还原

【Abstract】 [Objective]To establish a simple and rapid method for mercury ion detection.[Methods]Variations of D660nm/D530nm before and after adding mercury ion were compared through the spectroscopy of colloidal gold and DNA system which was scanned with a spectrophotometer to determine the content of mercury ion.[Results]The linear range of this method was0.1-2.0μg/L,the correlation coefficient(r) was 0.995,and the detection limit was 0.2μg/kg.[Conclusion]The method based on colloidal gold and DNA system is simple and rapid for mercury ion detection in food.更多还原