【摘要】 为了探索隐藏嗜酸菌(Acidiphilium cryptum)对多变极端矿山环境条件的感知和反应分子机制,预测和分析了隐藏嗜酸菌JF-5菌株的双组分信号转导系统(Two-component signal transduction system,TCS)的分布、结构及功能.鉴定了9对成对TCSs、2个杂合结构TCSs、3个孤儿组氨酸蛋白激酶(HK)和5个孤儿反应调节蛋白(RR);发现5个TCSs参与隐藏嗜酸菌对重金属响应转录调控;大多数HKs的N-末端具有接受信号的跨膜区、HAMP或PAS等结构域,RRs主要是OmpR亚家族,占总RRs的40%以上;从进化关系上来看,一些处在进化树同一分支上的共同聚簇TCS基因可能具有相同的进化途径.本研究结果可为研究隐藏嗜酸菌在极端环境中适应性分子机制提供新的方向.更多还原

【Abstract】 In order to explore the molecular mechanism of Acidiphilium cryptum response to extreme conditions in the mine environment,the distributions,structures and putative biological functions of two-component signal transduction systems(TCSs) in A.cryptum JF-5 are predicated and analyzed.And 9 TCSs,2 hybrid histidine kinases(HK),3 orphan HKs and 5 orphan response regulator proteins(RR) were identified.Five TCSs were involved in the transcription regulation of A.cryptum response to heavy metal.The most N-terminal regions of HKs were characterized by the presence of transmembrane helices,HAMPs,or PAS domains.More than 40 percent of putative RRs were classified into OmpR subfamily.From the evolutionary relationship,the common clustering TCS genes in the same branch of the evolutionary tree may have the same evolutionary pathway.Our results should open a novel research direction in A.cryptum for molecular mechanism of the adaptation in the extreme environment.更多还原