【摘要】 目的 观察艾司西酞普兰对抑郁大鼠海马BDNF表达及细胞凋亡的影响,探讨其作用机制。方法 40只雄性SD大鼠随机分为对照(A)、对照+药(B)、抑郁模型(C)和抑郁模型+药(D)组共4组,采用慢性不可预见性的温和刺激结合孤养法建立抑郁大鼠模型,TUNEL法检测海马细胞凋亡情况,实时荧光定量PCR方法检测海马BDNF、Bax、Bcl-2、Caspase 3 mRNA的表达。结果(1)C组海马细胞凋亡数显著增加,与A组比较差异有统计学意义(P<0.01),而B组细胞凋亡数与A组差异无统计学意义(P>0.05);D组细胞凋亡数明显减少,与C组比较差异有统计学意义(P<0.01)。(2)C组海马BDNF、Bcl-2 mRNA表达降低,Bax、Caspase 3 mRNA表达升高,与A组比较差异均有统计学意义(P<0.01);D组BDNF、Bcl-2 mRNA表达增加,Bax、Caspase 3 mRNA表达降低,与C组比较差异均有统计学意义(P<0.01)。结论 艾司西酞普兰可改善抑郁大鼠的抑郁行为及海马细胞凋亡,其机制可能是通过增加海马BDNF mRNA的表达,上调Bcl-2及下调Bax、Caspase 3 mRNA的表达,从而预防海马细胞凋亡而发挥脑保护的作用。更多还原

【Abstract】 Objective To explore the effect and mechanism of escitalopram on the expression of brain-derived neurotrophic factor(BDNF) and apoptosis in hippocampus of depression model rats.Methods Totals of 40 male SD rats were randomly divided into four groups:normal group(group A),normal group-treated with escitalopram(group B),model group(group C),and model group-treated with escitalopram(group D).Chronic mild unpredicted stress(CUMS) with solitary condition was taken to establish rat depression model.The open-field test and sucrose consumption were used to evaluate the depression behaviors of rats.TUNEL assay was used to determine neuronal apoptosis.The expression of mRNA of BDNF,Bax,Bcl-2 and Caspase 3 were detected by RT-PCR.Results(1) Compared with group A,the number of apoptotic cells in hippocampus was significantly increased in group C(P < 0.01),while group B had no significant difference(P > 0.05)(compared with group C,group D had a significant reduction in the number of apoptotic cells(P< 0.01).(2)Compared with group A,BDNF,Bcl-2 mRNA expression was decreased(P < 0.01),Bax and Caspase 3 mRNA expression was increased in group C(P < 0.01);while in group D,the expression of BDNF,Bcl-2mRNA was higher(P < 0.01),the expression of Bax and Caspase 3 mRNA was apparently compared with group C(P <0.01).Conclusions Escitalopram can improve depressive behavior and hippocampal apoptosis,and its mechanism may be caused by increasing BDNF mRNA expression,and promoting the protection of neurons,may be related to up-regulating of mRNA Bcl-2 expression and down- regulating of mRNA Bax,Caspase 3 expression,to decrease the neuronal apoptosis in hippocampal and play the role of brain protection.更多还原