【摘要】 目的比较免疫组化方法(IHC)和实时定量PCR(RT-PCR)对乳腺癌易感基因Her-2、EGFR、CK5/6在乳腺癌治疗和预后中的应用。方法对41例乳腺癌患者分别进行癌组织IHC和RT-PCR检测,测定癌组织Her-2、EGFR、CK5/6基因的过表达水平,分析比较两种方法检测的灵敏度和特异性。结果 41例乳腺癌患者均为女性,应用免疫组化方法,Her-2阳性表达21例,EGFR呈阳性表达8例,CK5/6阳性病例8例;应用RT-PCR方法,Her-2阳性表达27例,EGFR呈阳性表达21例,CK5/6阳性病例21例。其中Her-2应用两种方法所得阳性率没有统计学差别;EGFR、CK5/6应用两种方法所得阳性率不同,P<0.01。结论免疫组化方法与RT-PCR方法相比较,乳腺癌组织EGFR、CK5/6等基因在不同组织中的扩增和蛋白过表达具有差异性,Her-2不具有差异性,RT-PCR方法敏感性和准确性更高,但二者具有一致性。更多还原

【Abstract】 Objective To discuss the application in the treatment and prognosis of breast cancer with immunohistochemistry(IHC) and real-time quantitative PCR(RT-PCR) for breast cancer susceptibility gene Her-2、EGFR、CK5/6.Methods The Her-2,EGFR,CK5/6 gene expression levels were detected by IHC and RT-PCR in 41 patients with breast cancer.The sensitivity and specificity of two kinds of methods were analyzed and compared.Results Forty-one cases of breast cancer were women.Immunohistochemical methods showed 21 cases of Her-2 positive expression,8 cases of EGFR positive,and 8 cases of CK5/6-positive;But RT-PCR method showed Her-2 positive in 27 cases,EGFR positive in 21 cases,and CK5/6 positive in 21 cases.Her-2 positive rates were not statistically different with both of these methods(P>0.05);EGFR,CK5/6 positive rates of different methods were statistically significant(P<0.05).Conclusion Detection of EGFR and CK5/6 have difference in amplification and protein expression in different tissues,but Her-2 has not with two methods.RT-PCR method has higher sensitivity and accuracy,but their results are consistent.更多还原