【摘要】 将4种氮杂冠醚或吗啉取代的单Schiff碱锰(III)配合物作为仿水解酶模型催化α-吡啶甲酸对硝基苯酯(PNPP)水解。考察了单Schiff碱配体中取代基类型、氮杂冠醚取代的位置对其仿水解酶性能的影响;探讨了Schiff配合物催化PNPP水解的动力学和机理;提出了配合物催化PNPP水解的动力学模型。结果表明,在25℃条件下随着缓冲溶液pH值的增大,配合物催化PNPP水解速率提高,氮杂冠醚化单Schiff碱锰(III)配合物在催化PNPP水解反应中表现出良好的催化活性,Schiff碱配体结构显著影响配合物催化活性。更多还原

【Abstract】 Four mono-Schiff base manganese(III)complexes with either benzo-10-aza-crown ether pendants(MnL12Cl,MnL22Cl,MnL32Cl)or morpholino pendants(MnL42Cl)have been employed as models for hydrolase enzymes by studying the kinetics of their hydrolysis reactions with p-nitrophenyl picolinate(PNPP).The effects of the different ligands and the position of the aza-crown in the Schiff base complexes on the kinetics of PNPP hydrolysis were investigated.In addition,a kinetic mathematical model of PNPP cleavage catalyzed by these complexes was established.The results showed that the rate of the catalytic PNPP hydrolysis increased as the pH of the buffer solution increased.All four complexes exhibited high activity in the catalytic PNPP hydrolysis,which was influenced by the structures of the different Schiff base ligand.更多还原