【摘要】 目的比较肛门直肠常见病变组织中人乳头瘤病毒(HPV)感染的基因型分布情况及其临床意义。方法采用基因扩增结合基因芯片技术对302例肛门直肠常见病变组织标本进行23种HPV基因分型检测。结果 302例肛门直肠常见病变HPV总阳性率为40.73%(123/302),一重感染阳性率为27.48%(83/302),多重感染阳性率为13.25%(40/302),两性HPV总阳性率、一重和多重感染率比较差异无统计学意义(P>0.05)。结论基因扩增结合基因芯片检测技术可应用于肛门直肠组织HPV分型检测,对肛门直肠常见病变HPV感染的分子流行病学研究具有重要意义。更多还原

【Abstract】 Objective To compare the clinical distribution of human papillomavirus(HPV)genotypes in common anorectal lesions and its clinical significance.Methods Polymerase chain reaction and gene-chips technology were utilized for the detection of 23kinds of HPV genotypes in tissue specimens from 302cases of common anorectal lesions.And related materials of all subjects were analyzed.Results The total HPV positive rate was 40.73%(123/302)in all specimens.the positive rates of single genotype infection were 27.48%(83/302),the positive rates of multiple genotypes infection were 13.25%(40/302).The positive rates of the total HPV,single and multiple infection in two genders were not significantly different among these common anorectal lesions(P> 0.05).Conclusion PCR and gene chip technology can detect single and multiple HPV genotypes in tissue samples of common anorectal lesions with high sensitivity and specificity,especially fit for study on molecular epidemiology of HPV infection in tissues of common anorectal lesions.更多还原