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升麻多糖提取及其抗氧化作用
Extraction and Antioxidant Activity of Polysaccharides fromCimicifuga foetida
【摘要】 研究了升麻多糖的提取及其对羟基自由基的清除作用。采用热水浸提及醇沉法从升麻中提取多糖,通过比色法研究其清除羟基自由基的能力。升麻多糖清除能力与多糖浓度有明显的量效关系,清除50%羟基自由基所需要的多糖浓度为2.4mg/mL,结果表明升麻多糖具有良好的清除羟基自由基的能力。
【Abstract】 The extraction of polysaccharide fromCimicifuga foetidaand its effect of removal of hydroxyl radicals was studied.The crude Polysaccharide was extracted fromCimicifuga foetidausing hot water-ethanol method. The free radical cleaning effect was studied employing colorimentry method.The results showed that the polysaccharide had the ability to scavenge hydroxyl radicals, which had the dose-effect relationship with the concentration of polysaccharide that was 2.4mg/mL when scavenging 50% hydroxyl radicals.
【关键词】 升麻;
多糖;
提取;
羟基自由基;
【Key words】 Cimicifuga foetida; Polysaccharide; Extraction; Hydroxyl Radicals;
【Key words】 Cimicifuga foetida; Polysaccharide; Extraction; Hydroxyl Radicals;
【基金】 安徽省教育厅自然科学重点研究项目(KJ2007A123ZC);校教研项目(040-120006000008)
- 【文献出处】 光谱实验室 ,Chinese Journal of Spectroscopy Laboratory , 编辑部邮箱 ,2013年05期
- 【分类号】R285
- 【被引频次】23
- 【下载频次】308