【摘要】 对海产品中甜菜碱的检测条件进行了探讨。通过单因素和正交试验确定了水产品中甜菜碱提取的最佳条件为:络合物显色在20 min内进行比色测定,采用雷氏盐溶液10 mL、pH 1.0,10 000 r/min离心分离,检测波长525 nm。在此条件下检测了马氏珠母贝肉、马氏珠母贝粉、马氏珠母贝EAP粉、沙虫干、元贝干、生蚝等几种海产品甜菜碱的含量,结果表明,供试的几种海产品甜菜碱含量分别为:马氏珠母贝肉9.83(±0.004)mg/g,马氏珠母贝粉85.52(±0.002)mg/g,马氏珠母贝EAP粉84.29(±0.002)mg/g,沙虫干93.13±(0.003)mg/g,元贝干19.19(±0.013)mg/g,生蚝4.72(±0.003)mg/g。更多还原

【Abstract】 Using single factor and orthogonal experiments, the best conditions for betaine determination of marine products were discussed in this study. Betaine was extracted with 10 mL volume of saturated Reye salt solution(pH 1.0) and centrifuged by 10 000 r/min.The complex was determined by spectro-photometry at 525 nm wavelength in 20 min. Under this condition, betaine content of several marine products was detected, of which betaine content of dunker meat was 9.83(±0.004) mg/g, dunker powder 85.52(±0.002) mg/g, markov EAP nacre powder 84.29(±0.002) mg/g, worm dry 93.13±(0.003) mg/g, callops dry 19.19(±0.013) mg/g and oysters 4.72(±0.003) mg/g.更多还原