【摘要】 目的检测卡介苗免疫后小鼠脑组织匀浆液和血清中细胞因子的变化,为进一步研究新生期卡介苗接种影响小鼠中枢神经系统神经发生的分子机制研究做准备。方法实验分1周、2周、3周3个时间点,将新生24 h内的SPF级C57BL/6小鼠随机分为卡介组和对照组,每组12只,分别在新生24 h内背部皮下注射卡介苗和PBS。分别在生后1周、2周、3周采用摘眼球法采血,之后冰上无菌取海马组织并匀浆。用ELISA酶联免疫吸附法,分别测各时间点各实验组小鼠脑组织匀浆和血清中的IL-1β、IL-4、IL-10和TNF-α含量。结果 (1)与对照组相比,接种后1周、2周、3周时,卡介组小鼠海马中细胞因子IL-4和IL-10均多于对照组(P<0.05),血清中IL-4多于对照组(P<0.05);IL-10在第1周和第2周多于对照组(P<0.05)。与对照组相比,接种后1周、2周、3周时,卡介组小鼠海马中细胞因子IL-1β和TNF-α均少于对照组,血清中TNF-α少于对照组;IL-1β在第1周和第2周于对照组(P<0.05)。(2)同一时间点海马中的细胞因子含量远多于血清中含量。(3)随时间的推进,海马和血清中IL-4和IL-10有降低趋势;而小鼠海马和血清中IL-1β和TNF-α有增加趋势。结论新生期接种卡介苗成功后,可致小鼠海马和血清中的细胞因子浓度发生动态改变:与对照组相比,海马和血清中IL-4和IL-10的含量升高,且随时间有下降趋势,但IL-1β和TNF-α的含量降低,且随时间有增加趋势。更多还原

【Abstract】 Objective To detect the variation of cytokines in the hippocampal homogenate and serum of the neonatal mouse after BCG vaccination,and make the foundation for further studying the interactive molecular mechanism between BCG vaccination and neurogenesis in the central nervous system.Methods The experiment was practiced into three different time points,at the end of the first week,the second week and the third week.SPF C57BL/6 neonatal mice were randomly divided into 2 groups : ① a BCG group(B;total n=36,n=12 for each time-point).② a control group(C;total n=36,n=12 for each time-point).The BCG group received a BCG subcutaneous injection within 24 h after birth,while the control group received PBS.At the end of the first week,the second week and the third week,all of the mice were killed and got the serum and the hippocampus.The mice hippocampal homogenate were prepared immediately after the mice hippocampus removed from the ice.The cytokines in the mouse serum and hippocampal homogenate were detected with ELISA.Results ① Compared with the control group,at each time point,the concentration of IL-4 and IL-10 in mouse hippocampal homogenate of the BCG group were higher(P<0.05).But the concentration of IL-4 in mouse serum were higher(P<0.05);At the first and second week,the concentration of IL-10 were significantly higher in BCG group than in control group(P <0.05).At each time point,the concentration of IL-1β and TNF-α in mice hippocampal homogenate of the BCG group were less than the control group(P <0.05).In mouse serum,the concentration of TNF-α were less than the controls(P<0.05);and at the first and the second week,the concentration of IL-1β were less than the controls(P<0.05).② At each time point,the concentration of all the cytokines(IL-1β,IL-4,IL-10,TNF-α) were more in the mouse hippocampal homogenate than those in the mouse serum of both the BCG group and the control group.③ From the variation of cytokines in both of mouse serum and hippocampal homogenate of the BCG group and the control group,we found that,with the development of time,the concentration of IL-4 and IL-10 were decreased in both of the two samples ,while the concentration of IL-1β and TNF-α were increased.Conclusion The neonatal BCG vaccination may course a dynamically change in the concentration of cytokines.Compared with the control group,the concentration of IL-4 and IL-10 in both of mouse serum and hippocampal homogenate are higher,and with the development of time ,the concentration of them had a decling trend ,while the concentration of IL-1β and TNF-α are lower but had an increasing trend.更多还原