【摘要】 目的探讨丙泊酚对体外培养的新生大鼠大脑皮层星形胶质细胞(AST)合成凝血酶敏感蛋白1(THBS-1)的影响。方法采用出生后12 h SD大鼠进行大脑皮层AST分离培养及纯化,以不同浓度的丙泊酚(3、10、30、100、300μmol/L)和不同的暴露时间(6 h组、12 h组和24 h组)对体外培养的AST进行处理,观察AST合成THBS-1 mRNA和蛋白水平的变化。并以30μmol/L丙泊酚处理12 h,利用免疫细胞化学技术观察THBS-1在AST的分布。结果免疫荧光细胞化学检测显示丙泊酚处理组AST THBS-1表达水平明显升高(P<0.05),为空白对照组的2.3倍,高表达的THBS-1信号位于胞浆中。10、30、100μmol/L的丙泊酚可显著上调体外培养新生大鼠大脑皮层星形胶质细胞的THBS-1 mRNA和蛋白水平(P<0.01)。不同丙泊酚暴露时间组均可见THBS-1 mRNA和蛋白水平表达上调(P<0.05),尤以12 h组最为显著(P<0.01)。结论临床相关剂量的丙泊酚可以增加体外培养大鼠大脑皮层星形胶质细胞中THBS-1的表达。更多还原

【Abstract】 Objective To investigate the effect of propofol on the expression of thrombospondin-1(THBS-1) mRNA and protein in purified newborn rat cortical astrocytes in vitro.Methods Astrocytes were isolated from newborn rat cortex and grown in culture before exposure to propofol at 3,10,30,100 or 300 μmol/L for 6 h,12,or 24 h.The mRNA level of THBS-1 was detected by RT-PCR,and the protein level of THBS-1 was detected by immunofluorescence cytochemistry and Western blotting.Results Propofol exposure caused significantly upregulated THBS-1 level in cultured astrocytess(P<0.05) to a level about 1.3 times higher than that in control cells.The mRNA and protein levels of THBS-1 in cultured rat cortical astrocytes were upregulated by exposures to 10,30 and 100 μmol/L propofol(P<0.01).High expression of THBS-1 mRNA and protein was detected in the cells with exposures for different durations(P<0.05),especially in the 12 h group(P<0.01).Conclusion Propofol at clinically relevant concentrations can modulate the level of THBS-1 secreted by astrocytes of rat cerebral cortex in vitro.更多还原